骨髓间充质干细胞对染矽尘小鼠肺部早期炎症抑制作用

发布时间：2018-01-08 02:30

本文关键词：骨髓间充质干细胞对染矽尘小鼠肺部早期炎症抑制作用　出处：《中国职业医学》2016年02期 　论文类型：期刊论文

【摘要】：目的研究骨髓间充质干细胞(BMSCs)移植对染矽尘小鼠肺部早期炎症的影响。方法取5只无特定病原体级健康雄性C57BL/6小鼠,采用全骨髓贴壁法分离培养BMSCs。取30只同类小鼠随机分为对照组、二氧化硅组和BMSCs移植组3组,以气管暴露法建立动物模型,对照组小鼠予气管内一次性注射0.90%氯化钠溶液20.0μL;二氧化硅组和BMSCs移植组小鼠先予气管内一次性注射20.0μL质量浓度为250 g/L的二氧化硅混悬液,6 h后再分别经尾静脉输注500.0μL的0.90%氯化钠溶液或细胞密度为1×109/L的BMSCs悬液。造模后第7天处死小鼠,观察肺组织病理学改变情况,采用酶联免疫吸附实验检测小鼠血清中白细胞介素(IL)-1β、IL-6和IL-10水平,采用实时定量聚合酶链反应仪检测小鼠肺组织中上述细胞因子mRNA的表达情况。结果 BMSCs表面分子分化抗原(CD)29、CD34、CD90、CD105、CD106的阳性率分别为67.70%、0.12%、39.00%、37.10%和20.10%。肺组织病理学观察显示,二氧化硅组小鼠肺泡壁增厚,肺泡间隔增宽,肺泡结构破坏;BMSCs移植组肺组织结构无明显破坏,肺泡腔大小均匀,肺泡结构完整。二氧化硅组小鼠血清中IL-1β、IL-6水平以及肺组织中IL-1β、IL-6 mRNA相对表达水平分别高于对照组和BMSCs移植组(P0.05),BMSCs移植组小鼠血清中IL-1β水平和肺组织中IL-1βmRNA相对表达水平均高于对照组(P0.05)。各组小鼠血清中IL-10水平和肺组织中IL-10 mRNA相对表达水平分别比较,差异均无统计学意义(P0.05)。结论 BMSCs可通过下调促炎因子IL-1β和IL-6的表达减轻染矽尘小鼠肺部早期炎性损伤。
[Abstract]:Objective to study the bone marrow mesenchymal stem cells (BMSCs) transplantation on the effect of silica exposed lungs of mice early inflammation. Methods 5 specific pathogen free male C57BL/6 mice, BMSCs. were isolated and cultured from 30 similar mice were randomly divided into control group using whole bone marrow adherent method. Two silica group and BMSCs transplantation 3 groups, to establish the animal model of trachea was exposed and control mice were treated with intratracheal injection of 0.90% Sodium Chloride Solution 20 L; silica group and BMSCs transplantation group were first to two silicon oxide intratracheal injection of 20 L concentration of 250 g/L suspension, 6 h respectively after infusion through tail vein note 500 L 0.90% or Sodium Chloride Solution cell density of 1 * 109/L BMSCs suspension. The mice were sacrificed seventh days after modeling, pathological changes in lung tissues were observed, using enzyme-linked immunosorbent assay in mouse serum interleukin (IL) -1 Beta, IL-6 and IL-10 level, the expression of the cytokines of mRNA in lung tissue of mice was detected by real-time quantitative polymerase chain reaction. The results of BMSCs molecular surface differentiation antigen (CD) 29, CD34, CD90, CD105, CD106 positive rate was 67.70%, 0.12%, 39%, 37.10% and 20.10%. observed in lung tissue the pathology of silica mice alveolar wall thickening, alveolar septal thickening, alveolar structural damage; BMSCs transplantation group, lung tissue structure without obvious damage, alveolar cavity size uniform, the alveolar structure integrity. Serum silica group IL-1 beta, IL-6 and IL-1 in lung beta, IL-6 mRNA relative expression levels were higher than those in control group and BMSCs transplantation group (P0.05), BMSCs transplantation group mice serum IL-1 level and lung tissue in IL-1 beta mRNA relative expression levels were higher than the control group (P0.05). The level of serum IL-10 and IL-10 in lung mRNA There was no significant difference in relative expression level (P0.05). Conclusion BMSCs can alleviate early inflammatory injury in mice exposed to silica dust by down regulating the expression of proinflammatory cytokines IL-1 beta and IL-6.

【作者单位】：山西医科大学公共卫生学院;广东省职业病防治院广东省职业病防治重点实验室;南方医科大学基础医学院免疫学教研室;
【基金】：国家科技支撑计划项目(2014BAI12B01) 国家临床重点专科建设项目(2011-09) 广东省职业病防治重点实验室(2012A061400007) 国家自然科学基金(81202246) 广东省科技计划项目(2014A020212551) 广东省医学科研基金(A2015624)
【分类号】：R135.2
【正文快照】： 510300;3.南方医科大学基础医学院免疫学教研室,510515矽肺是由于长期吸入大量游离二氧化硅粉尘所引起的以肺部广泛结节性纤维化为主的疾病,病程分为肺泡炎期和纤维化期,矽尘所致肺泡炎是其导致肺纤维化的病理基础[1]。目前临床上矽肺常用治疗药物为克矽平,但其不能治愈疾病且

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