汽车喷漆涂料挥发性有机物对小鼠肝脏的损伤作用

发布时间：2018-01-14 00:06

本文关键词：汽车喷漆涂料挥发性有机物对小鼠肝脏的损伤作用　出处：《环境与职业医学》2015年11期 　论文类型：期刊论文

【摘要】：[目的]研究汽车喷漆涂料挥发性有机物对小鼠肝脏的损伤作用,为了解此类涂料的安全性提供基础。[方法]清洁级昆明种雄性小鼠78只,随机分为13组,每组6只。4种汽车喷漆涂料分别分为低、中、高三个染毒剂量组(染毒终浓度分别为底漆:320、600、1110 mg/m3;面漆:300、580、1 080 mg/m3;清漆:350、680、1 280 mg/m3;黑漆:400、580、1 350 mg/m3),用静式呼吸道染毒法染毒,每天染毒2 h;对照组放入无喷漆涂料的染毒柜2 h;持续28 d。实验期结束后,进行肝功能血液生化检测,并取肝制备病理切片。[结果]4种涂料挥发性有机物中均含有苯和苯系物、烯烃、烷烃等有机成分。肝病理切片观察表明,4种油漆不同剂量染毒后小鼠肝细胞均出现不同程度的损伤,高剂量组细胞损伤较严重。各染毒组小鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)含量较对照组均有不同程度增加,但剂量反应关系不明显。黑漆各染毒组ALT含量均高于对照组,中、高剂量组AST、ALP含量高于对照组(P0.05),低剂量组与对照组相比差异无统计学意义(P0.05);面漆各染毒组ALT、ALP含量均高于对照组(P0.05),高剂量组ALT、AST含量高于低剂量组和中剂量组,高剂量组ALP含量高于低剂量组(P0.05);清漆各染毒组ALT、AST、ALP含量均高于对照组(P0.05),ALP含量随着染毒剂量的升高而增加(P0.05);底漆各染毒组ALT含量均高于对照组,AST、ALP含量仅高剂量组差异有统计学意义(P0.05)。不同涂料同一剂量组间比较,清漆对肝功能影响较大。[结论]汽车喷漆涂料挥发性有机物对小鼠肝脏有明显的肝毒性,并且染毒剂量越大毒性越明显。
[Abstract]:[Objective] to study the car paint coating of volatile organic compounds on liver injury in mice, in order to understand the safety of such coatings provide the basic method.] clean level 78 male Kunming mice, were randomly divided into 13 groups, 6 rats in each group.4 car lacquer coatings were divided into low, high dose of three group (at final concentrations of primer: 3206001110 mg/m3; 300580,1 080 mg/m3; varnish finish: 280: 350680,1 mg/m3; black paint: 400580,1 350 mg/m3), with static respiratory tract exposure exposure, daily exposure to 2 h; the control group in the non exposure cabinet painting coating 2 h; after the experimental period lasted for 28 d. The blood biochemical test, liver function, pathological results.]4 prepared for coating of volatile organic compounds contain benzene and benzene, olefin and liver, alkane organic components. Liver histopathological observation showed that 4 kinds of paint is not the same dose after exposure of mouse liver Cells were damaged at different degrees, the high dose group of cell injury is more serious. All groups of mice serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) content compared with the control group showed different degrees of increase, but the dose-response relationship was not obvious. Black paint groups were ALT higher than the control group, high dose group, AST, ALP were lower than the control group (P0.05), low dose group had no significant difference compared with the control group (P0.05); finish groups ALT, ALP were higher than that of the control group (P0.05), high dose of ALT group, AST was higher than that in low dose group and medium dose group, high dose group of ALP was higher than that of low dose group (P0.05); VARNISHES groups ALT, AST, ALP content were higher than the control group (P0.05), the content of ALP increased with the increase of the doses (P0.05); the content of ALT primer exposed groups were higher than those in control group, AST, ALP content only high dose The difference was statistically significant (P0.05). The same dose of different coating groups, affect varnish on liver function. Conclusion: the car paint coating of volatile organic compounds showed significant hepatic toxicity on mice liver, and the dose the greater the toxicity is more obvious.

【作者单位】：华北理工大学公共卫生学院实验中心河北省煤矿卫生与安全实验室;唐山市环保局;北京工业大学环境与能源工程学院环境科学系;
【基金】：国家环保部项目(编号:201209003)
【分类号】：R114
【正文快照】： 面漆,黑漆是黑色的面漆;清漆是为了增加面漆的光泽度以及硬度而喷涂在面漆之上的一种无色透明的液体。4种汽车喷漆涂料VOCs各种成分含量由北京工业大学环境与能源工程学院解析。实验动物为清洁级昆明种小鼠78只,雄性,体重18~22 g,购自北京维通利华实验动物技术有限公司,许可证

【参考文献】