四棱豆功能成分及特性研究

发布时间：2018-01-15 21:07

本文关键词：四棱豆功能成分及特性研究　出处：《湖南农业大学》2012年硕士论文　论文类型：学位论文

【摘要】：四棱豆是一种叶片、果实均可食用的新型粮、油、菜、药兼用型的经济作物,目前已经在我国南方推广种植,本研究对四棱豆的功能成分及其特性进行了初步研究,为开发四棱豆营养保健品提供理论基础。本研究利用原子荧光光谱法、紫外分光光度法、荧光分光光度计法对四棱豆的九个品种：湘棱豆一号、湘棱豆二号、湘棱豆三号、坪阳一号、中翼一号、Z4、 K00002、K000026、K000032的硒含量、维生素E含量和维生素C含量进行了检测；采用(1,1-二苯基-2-三硝基苯肼)DPPH自由基清除法对九个品种的四棱豆抗氧化能力做出了评价,并对湘棱豆的提取方式进行了优化选择。结果表明,湘棱豆系列的Vc含量为13.09±0.31mg/100g, Ve含量为128.71+3.47mg/100g,硒含量为7.92±0.64μg/g。在保温10min条件下的清除率为：湘棱豆一号为37.82%、湘棱豆二号为40.51%、湘棱豆三号35.67%。采用不同溶剂提取湘棱豆系列后,对DPPH自由基的清除效率为石油醚粗提物乙酸乙酯粗提物丙酮粗提物水粗提物乙醇粗提物。建立低硒大鼠模型,采取灌胃的方式对大鼠补充高低两个剂量组的亚硒酸钠、富硒酵母和四棱豆硒蛋白溶液,研究不同硒源对大鼠生长发育、脏器系数、硒含量、抗氧化活力等生理指标的影响。将大鼠随机分成6个处理组,每组8只,进行为期60d的生存试验。试验结果表明：各处理组大鼠与空白组大鼠的肝脏系数均有显著性差异(P0.05),四棱豆硒蛋白高剂量组大鼠体重与空白组有显著差异(P0.05)；各处理组大鼠硒含量、SOD活性、MDA含量、GSH-px活性和TrxR活性均与对照组有显著性差异(P0.05),其中亚硒酸钠组大鼠组织硒含量与富硒酵母组和四棱豆硒蛋白组有显著性差异(P0.05),四棱豆硒蛋白高剂量组的血清和肝脏SOD活力提升高达17.7%和21.2%,而四棱豆硒蛋白和富硒酵母两个剂量组的GSH-px和TrxR活性均与亚硒酸钠组有显著差异(P0.05)。以高血脂大鼠为试验模型,采用湘棱豆乙醇提取物三个剂量组灌胃处理高血脂大鼠,并用辛伐他汀药物作为阳性对照组,考察大鼠的生长情况、SOD酶活、MDA含量、血清转氨酶活力和脂质代谢情况。结果表明,湘棱豆提取液浓度为7mg/mL组抗氧化效果最明显,体重较造模时有一定下降,WBEE(四棱豆乙醇提取物)高剂量组的肾周脂和睾丸周脂相对于高血脂模型组下降了22.22%和18.28%,肝脏重量也降低为12.76g,H-WBEE的肝脏匀浆MDA含量下降了13.43%,H-WBEE组谷丙转氨酶和谷草转氨酶分别下降了13.6%和11.7%,TG、 TC、LDL-c含量分别下降了36.2%、23、4%.66.7%, HDL-c含量增加了35.1%。结果表明,湘棱豆品系的Vc、Ve、硒含量和自由基的清除率均高于其他品种,采用乙醇提取湘棱豆的粗提物对DPPH自由基的清除率高于其他提取剂。四棱豆硒蛋白组的抗氧化活性高于亚硒酸钠组和富硒酵母组,而以乙醇提取后的WBEE高剂量组能明显改善大鼠肥胖和降血脂,与降脂药物效果相差不大。
[Abstract]:Tetragonia bean is a new kind of cash crop with edible fruit and edible grain, oil, vegetable and medicine. It has been popularized and planted in the south of China. In this study, the functional components and characteristics of tetragonia bean were studied preliminarily, which provided a theoretical basis for the development of nutrition and health products of quadrangular bean. In this study, atomic fluorescence spectrometry, ultraviolet spectrophotometry and fluorescence spectrophotometer were used to study nine varieties of tetragonia: Xianglingdou 1, Xianglingdou 2, Xiangleng bean 3, Pingyang 1 and Zhongyi 1. The selenium content, vitamin E content and vitamin C content of Z4, K00002, K000026 and K000032 were determined. The antioxidant ability of nine varieties of tetragonia bean was evaluated by DPPH free radical scavenging method of 1-diphenyl-2-trinitrophenylhydrazide. The results showed that the VC content of Xiangleng bean was 13.09 卤0.31 mg / 100 g. The content of ve was 128.71 3.47 mg / 100 g. The selenium content was 7.92 卤0.64 渭 g / g. The clearance rate of Xianglingdou 1 and Xianglingdou 2 was 37.82 and 40.51% respectively. The scavenging efficiency of DPPH free radical was: crude petroleum ether extract, ethyl acetate extract, acetone extract, ethanol crude extract. The rat model of low selenium was established, and sodium selenite, selenium enriched yeast and tetragonium bean selenium protein solution were added to the rats by gavage to study the growth and development of rats with different selenium sources and viscera coefficient. The effect of selenium content, antioxidant activity and other physiological indexes. The rats were randomly divided into 6 treatment groups, 8 rats in each group. The survival test was conducted for 60 days. The results showed that the liver coefficients of each treatment group and blank group were significantly different (P 0.05). There was significant difference in body weight between the high dose group and the blank group (P 0.05). Se content sod activity MDA content GSH-px activity and TrxR activity of rats in each treatment group were significantly different from those of the control group (P0.05). The content of selenium in sodium selenite group was significantly different from that in selenium enriched yeast group and tetrahedral bean protein group (P0.05). The activity of SOD in serum and liver was increased by 17.7% and 21.2% in the high dose group of selenoprotein. The activities of GSH-px and TrxR in the selenoprotein and selenium-enriched yeast groups were significantly different from those in the sodium selenite group (P 0.05). Taking hyperlipidemia rats as the experimental model, three dose groups of ethanol extract of Xiangleng bean were used to treat rats with hyperlipidemia, and Simvastatin was used as positive control group to investigate the growth of rats and the activity of SOD. The content of MDA, the activity of serum aminotransferase and the metabolism of lipid. The results showed that the antioxidation effect was the most obvious in the group of 7mg / mL extract of Xiangleng bean, and the body weight was lower than that of the model. Compared with the hyperlipidemia model group, the perirenal lipid and testicular pericarpium decreased by 22.22% and 18.28, and the liver weight decreased to 12.76g in the high dose group. The content of MDA in liver homogenate of H-WBEE decreased by 13.43% and decreased by 13.6% and 11.7T respectively in H-WBEE group. The content of LDL-c decreased 36.2%, and the content of HDL-c increased 35.1g. The results showed that the content of selenium and the scavenging rate of free radical in Xiangleng bean were higher than those in other varieties. The scavenging rate of DPPH free radical of the crude extract extracted by ethanol was higher than that of other extracts, and the antioxidant activity of selenoprotein group was higher than that of sodium selenite group and selenium-enriched yeast group. However, the high dose group of WBEE extracted with ethanol could obviously improve obesity and reduce blood lipid in rats, and the effect was not different from that of antilipidemic drugs.
【学位授予单位】：湖南农业大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R151

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