中国主要水果抑制肝癌HepG2细胞和结肠腺癌Caco-2细胞增殖活性评价

发布时间：2018-01-15 22:11

本文关键词：中国主要水果抑制肝癌HepG2细胞和结肠腺癌Caco-2细胞增殖活性评价　出处：《现代食品科技》2015年04期 　论文类型：期刊论文

【摘要】：分别采用Folin-Ciocalteu法测定了水果提取物的总酚含量,采用亚甲基蓝法测定了其抗人肝癌细胞Hep G2和人结肠腺癌细胞Caco-2增殖的活性,分析了总酚含量与抗Hep G2和Caco-2细胞增殖活性之间的相关性。结果显示,25种水果中李子的总酚含量最高(1686.08±96.94μmol GAE/100鲜果),西瓜(83.54±2.10μmol GAE/100 g鲜果)和哈密瓜(79.35±0.76μmol GAE/100 g鲜果)的总酚含量最低;在可测出抗增殖EC50值的水果中,李子(18.99±0.06 mg/m L)和番石榴(20.94±0.09 mg/m L)抗Hep G2细胞增殖的活性最强,梨的活性最弱(389.63±10.82 mg/m L)。李子抗Caco-2细胞增殖的活性最强(8.73±0.11 mg/m L),火龙果的活性最弱(388.07±7.04 mg/m L)。水果的抗Hep G2和Caco-2细胞增殖活性与其总酚含量相关性显著(R2=0.4147,p0.01;R2=0.4071,p0.05),该相关性表明水果中的多酚具有良好的抗肿瘤细胞增值的活性。
[Abstract]:The content of total phenols in fruit extracts was determined by Folin - Ciocalteu method . The correlation between total phenol content and proliferation activity of human colon adenocarcinoma cell line Caco - 2 was determined by methylene blue method . The results showed that the total phenol content of 25 kinds of fruits was the highest ( 16.86 . 08 卤 96.94 渭mol / 100 g fresh fruit ) , watermelon ( 83.54 卤 2.10 渭mol / m L ) and Guava ( 79.35 卤 0.76 渭mol / m L ) had the strongest activity , and the activity of pear was the weakest ( 389.63 卤 10.82 mg / ml ) . The activity of Li Zi against Caco - 2 cells was strongest ( 8.73 卤 0.11 mg / ml ) , and the activity of pitaya was the weakest ( 388.07 卤 7.04 mg / ml ) . There was a significant correlation between the proliferation activity and the total phenol content of the fruits ( R2 = 0.4147 , p0.01 ; R2 = 0.4071 , p0.05 ) . The correlation showed that the polyphenol in fruit had good activity of anti - tumor cell proliferation .

【作者单位】：深圳职业技术学院深圳市发酵精制检测系统重点实验室;
【基金】：广东省自然科学基金资助项目(S2011010004455) 深圳市科技计划项目(JC201005280530A,JCYJ20130331151222011) 广东省高等职业院校珠江学者岗位计划资助项目(2011)
【分类号】：R151;TS201.4
【正文快照】： 流行病学调查表明,膳食方式与慢性疾病如癌症、心血管病和早老性痴呆症有密切联系,而膳食摄入充足的水果可以有效减少这些慢性疾病的发病风险[1~2]。进一步的实验研究证实,水果中多酚类物质和Vc通过抗氧化、调节免疫、调控细胞增殖和凋亡、调控癌基因和抑癌基因表达、调节激素

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