低温空气等离子体对金黄色葡萄球菌杀灭效果与机理研究

发布时间：2018-01-18 00:23

本文关键词：低温空气等离子体对金黄色葡萄球菌杀灭效果与机理研究　出处：《中国消毒学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的利用低温空气等离子体反应装置,对模拟染菌载体聚氯乙烯(PVC)、聚四氟乙烯(PTFE)和聚对苯二甲酸乙二醇酯(PET)表面金黄色葡萄球菌的杀灭效果和机理进行研究。方法采用双悬浮探针对空气等子体的电子温度以及电子自旋共振波谱对自由基浓度进行了测定;采用扫描电子显微镜观察等离子体处理前后细菌细胞的形态变化。结果空气等离子体的灭菌效果符合放电区余辉区远程区的趋势;不同载体材料表面灭菌效果顺序与材料的亲水性一致;对数生长期的菌体比稳定期更容易被等离子体杀灭;有机污染物浓度增大,灭菌效果减弱;扫描电镜结果证实细菌细胞不同程度损伤,内容物逸出。结论等离子体灭菌过程是由各种活性粒子协同作用对细菌细胞造成破损的结果,而自由基在其中起主导作用,紫外光仅起辅助作用。
[Abstract]:Objective to study the effect of polyvinyl chloride (PVC) on the simulated bacterial carrier polyvinyl chloride (PVC) by using a low temperature air plasma reactor. Polytetrafluoroethylene teflon (PTFEE) and polyethylene terephthalate (PET). The killing effect and mechanism of Staphylococcus aureus on surface were studied. Methods the electron temperature and electron spin resonance spectroscopy were used to determine the concentration of free radical. The morphological changes of bacterial cells were observed by scanning electron microscope before and after plasma treatment. Results the sterilization effect of air plasma was in line with the trend of the remote region in the afterglow region of the discharge region. The order of sterilizing effect on the surface of different carrier materials is consistent with the hydrophilicity of the materials. The bacteria in logarithmic growth period are more likely to be killed by plasma than those in stable phase. When the concentration of organic pollutants increased, the sterilization effect was weakened. The results of scanning electron microscope showed that the bacterial cells were damaged and the contents escaped. Conclusion the process of plasma sterilization is caused by the synergistic effect of various active particles on the bacterial cells. Free radicals play a leading role and ultraviolet light only plays an auxiliary role.
【作者单位】：陕西省疾病预防控制中心;西安工业大学材料与化工学院;
【基金】：国家自然科学基金(21501139)
【分类号】：R187
【正文快照】： 金黄色葡萄球菌属于微球菌科葡萄球菌属,是引起化脓性疾病的重要致病菌[1,2]。耐药性金黄色葡萄球菌的产生,为感染性疾患治疗带来极大困难,迫切需要一些安全、简便、快速杀菌且无毒性残留的消毒杀菌方法。有报道直流脉冲能够有效地杀灭细菌和酵母菌,等离子体灭菌技术作为一门

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