角鲨烯对小鼠急性酒精性肝损伤的保护作用

发布时间：2018-01-18 12:20

本文关键词：角鲨烯对小鼠急性酒精性肝损伤的保护作用　出处：《食品工业科技》2015年16期 　论文类型：期刊论文

【摘要】：目的:研究角鲨烯对急性酒精性肝损伤的保护作用。方法:60只ICR小鼠随机分为正常组,模型组,阳性组,角鲨烯高、中、低剂量组。正常组和模型组灌胃等体积花生油,阳性组灌胃葵花牌护肝片350 mg/kg;角鲨烯高、中、低剂量组分别灌胃角鲨烯500、250、125 mg/kg,每日1次,30 d后制备急性酒精性肝损伤模型。模型制备16 h后检测肝脏系数,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和甘油三酯(TG)水平及肝脏丙二醛(MDA)和还原型谷胱甘肽(GSH)含量。结果:角鲨烯可明显降低血清ALT、AST、TG水平,降低肝组织MDA含量,并提高肝组织GSH含量,且呈明显的剂量依赖性,大剂量还能明显降低肝损伤小鼠的肝脏系数。结论:角鲨烯对小鼠急性酒精性肝损伤具有明显的保护作用,其机制可能与其抗氧化作用有关。
[Abstract]:Objective: to study the protective effect of squalene on acute alcoholic liver injury. Methods: 60 ICR mice were randomly divided into normal group, model group, positive group, high squalene group, middle squalene group. In the low dose group, the normal group and the model group were fed with peanut oil of the same volume, and the positive group were given the Kuihua Hugan tablet 350 mg / kg; Squalene was given to 500,250,125 mg / kg squalene once a day in high, medium and low dose groups. After 30 days, the acute alcoholic liver injury model was established. The liver coefficient and serum alanine aminotransferase (alt) were measured 16 hours after the establishment of the model. The levels of glutamic oxaloacetic transaminase (AST) and triglyceride (TG) and the contents of malondialdehyde (MDAs) and reduced glutathione (GSH) in liver were significantly decreased by squalene. TG level decreased the content of MDA in liver tissue and increased the content of GSH in liver tissue in a dose-dependent manner. Conclusion: squalene has obvious protective effect on acute alcoholic liver injury in mice and its mechanism may be related to its antioxidation effect.
【作者单位】：浙江省医学科学院;浙江海力生研究院;
【基金】：国家“重大新药创制”科技重大专项(2013ZX09303005) 浙江省医学重点学科群项目(XKQ-010-001)
【分类号】：R151
【正文快照】： 角鲨烯(squalene)又名鲨烯,于1906年首次从鲨鱼肝脏中分离得到,化学名为2,6,10,15,19,23-六甲基-2,6,10,14,18,22-二十四碳六烯,是一种高度不饱和烃类化合物[1]。角鲨烯具有增强机体免疫能力、抗氧化、抗衰老、抗疲劳、抗肿瘤等多种生理功能,常被添加到功能性食品和化妆品中[2

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