碱提醇沉黑木耳多糖体外和体内降血脂功能

发布时间：2018-01-20 13:59

本文关键词： 黑木耳多糖胆酸盐降血脂卵磷脂胆固醇酰基转移酶　出处：《食品科学》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨黑木耳多糖体内和体外降血脂功能。方法:采用离体实验模拟人体胃和肠道的p H值环境,探讨不同溶剂提取黑木耳多糖及其不同醇沉片段对胆酸盐的结合能力,评价其体外降血脂功能。以高脂饲料喂养小鼠建立高血脂模型,通过黑木耳多糖的治疗,比较治疗前后小鼠体质量、血清总胆固醇(total cholesterol,TC)、总甘油三酯(triglyceride,TG)、低密度总胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度总胆固醇(high density lipoprotein cholesterol,HDL-C)含量及其关键酶卵磷脂胆固醇酰基转移酶(lecithin cholesterol acyltransferase,LCAT)、肝脂酶(liver lipase,HL)和脂蛋白脂酶(lipoprotein lipase,LPL)的活性,研究碱提黑木耳多糖对高血脂模型小鼠的降血脂作用。结果:体外降血脂实验结果表明:3种不同的黑木耳多糖样品中,碱提黑木耳多糖的结合效果最好;不同体积分数乙醇醇沉黑木耳多糖片段的结合效果比较显示,70%醇沉片段结合胆酸盐效果最好。体内降血脂实验结果发现,碱提+70%醇沉黑木耳多糖能够缓解小鼠体质量的增长,显著缓解TC、TG和LDL-C含量的下降,显著提升HDL-C水平,控制LCAT含量、HL和LPL活性的降低。结论:碱提+70%醇沉黑木耳多糖具有降血脂功能。
[Abstract]:Objective: To study the hypolipidemic function of Auricularia polysaccharide in vivo and in vitro. Methods: the in vitro simulated human gastric and intestinal P H value, extraction of Auricularia polysaccharide and different alcohol binding ability of bile salts to fragments of different solvents, evaluate its in vitro hypolipidemic function. With a high fat diet fed mice establish the model of hyperlipidemia, through the treatment of Auricularia polysaccharide, the body mass of mice before and after treatment, serum total cholesterol (total, cholesterol, TC), total triglyceride (triglyceride, TG), low density cholesterol (low density lipoprotein cholesterol, LDL-C), high density cholesterol (high density lipoprotein cholesterol, HDL-C) content and the key enzyme of lecithin cholesterol acyltransferase (lecithin cholesterol, acyltransferase, LCAT), hepatic lipase (liver lipase, HL) and lipoprotein lipase (lipoprotein lipase, LPL) activity of alkali The hypolipidemic effects of extract of Auricularia polysaccharide on hyperlipidemia model mice. Results: in vitro hypolipidemic experimental results show that 3 kinds of Auricularia polysaccharide samples, combined with the best effect of alkali extraction of Auricularia polysaccharide; different volume fraction of ethanol of Auricularia polysaccharide fragments with effect shows 70% alcohol fragment binding bile salts. The best in vivo hypolipidemic experimental results showed that the precipitation of Auricularia polysaccharide can reduce the body weight of mice +70% alcohol alkali extraction and growth, significantly reduced TC, decreased TG and LDL-C content, significantly enhance the level of HDL-C, control LCAT content, decreased HL and LPL activity. Conclusion: Shen black fungus polysaccharide has the function of reducing blood lipid alkali +70% alcohol.

【作者单位】：东北林业大学林学院;通化师范学院制药与食品科学学院;中国农业科学院农产品加工研究所主食加工技术研究院;聊城大学农学院;哈尔滨工业大学化工与化学学院;
【基金】：黑龙江省应用技术研究与开发计划项目(GA13B202) 中央高校基本科研业务费专项(DL11BA18)
【分类号】：R151
【正文快照】： 近年来,心脑血管疾病已成为危害人群健康最严重的疾病。据流行病学报告,在我国每小时约300人死于心脑血管疾病,而且发病率呈逐年上升的趋势,并且该病症的发病年龄趋于年轻化[1]。而脂质代谢与心脑血管疾病密切相关,脂质代谢紊乱所致的动脉粥样硬化是引起心脑血管疾病发生、发

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