DEHP亚慢性暴露对大鼠精子运动参数影响

发布时间：2018-01-21 15:57

本文关键词： 邻苯二甲酸(-乙基已基)酯(DEHP) 精子计算机辅助精子分析系统(CASA)　出处：《中国公共卫生》2017年04期 　论文类型：期刊论文

【摘要】：目的观察邻苯二甲酸(2-乙基已基)酯(DEHP)染毒90 d对大鼠精子运动能力的毒性作用。方法将健康清洁级SD雄性大鼠40只随机分为对照组和3个DEHP染毒组(5、50、500 mg/kg),进行90 d经口染毒;取睾丸、附睾称重,并用扩散法收集大鼠附睾尾精子,应用计算机辅助精子分析系统(CASA)对精子的运动参数进行检测。结果 5 mg/kg DEHP组大鼠精子直线性(LIN)为(44.00±2.52)%,明显低于对照组(50.00±2.71)%(P0.05);500 mg/kg DEHP组大鼠精子鞭打频率(BCF)为(5.8±4.2)Hz,明显低于对照组(10.4±1.7)Hz(P0.05);与对照组比较,500 mg/kg DEHP组大鼠精子的平均路径速度(VAP)、直线运动速度(VSL)、曲线运动速度(VCL)、精子头侧摆幅度(ALH)、BCF明显下降,差异均有统计学意义(均P0.05);随着DEHP染毒浓度增加,大鼠精子各项运动参数逐渐降低,精子的运动能力下降。结论 DEHP对大鼠附睾精子的运动能力有直接毒性作用。
[Abstract]:Objective to observe the content of 2-ethylhexyl phthalate (DEHP). Methods Forty SD male rats of clean grade were randomly divided into two groups: control group and 3 DEHP groups. 50,500 mg / kg, administered orally for 90 days; Testis were taken and epididymis was weighed and sperm was collected from rat epididymal tail by diffusion method. The sperm motility parameters were measured by computer aided sperm analysis system (CASA). Results the sperm linear line in 5 mg/kg DEHP group was (. 44.00 卤2.52%. It was significantly lower than that in the control group (50.00 卤2.71). The frequency of sperm whipping in the 500 mg/kg DEHP group was 5.8 卤4.2 Hz, which was significantly lower than that in the control group (10.4 卤1.7). Compared with the control group, the average path velocities of spermatozoa, linear motion velocities and curvilinear motion velocities were observed in the #number0# mg/kg DEHP group. The amplitude of head and side pendulum of spermatozoa decreased significantly (P 0.05). With the increase of the concentration of DEHP, the motility of sperm decreased and the motility of sperm decreased. Conclusion DEHP has a direct toxic effect on the motility of rat epididymal spermatozoa.
【作者单位】：东南大学公共卫生学院;江苏省疾病预防控制中心毒理与功能评价所;
【基金】：东南大学环境医学工程教育部重点实验室开放课题(2015EME002) 中央高校基本科研业务费专项资金资助江苏省普通高校研究生科研创新计划(SJZZ15_0026)
【分类号】：R114
【正文快照】： 邻苯二甲酸(2-乙基已基)酯[di-(2-ethylhex-yl)phthalate,DEHP]属于邻苯二甲酸酯类化合物(phthalic acid ester,PAE),是目前应用最广泛的增塑剂之一。由于DEHP与产品基质间为非共价结合,随着时间推移,DEHP容易从产品中释放到周围环境造成污染,并通过饮水、摄食和呼吸等多种途

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