甘氨酸对果糖饮食诱导大鼠肠源性内毒素血症的保护作用

发布时间：2018-01-24 02:13

本文关键词： 果糖饮食甘氨酸肠源性内毒素血症肠型碱性磷酸酶肠黏膜屏障　出处：《现代预防医学》2015年16期 　论文类型：期刊论文

【摘要】：目的探讨果糖饮食致大鼠发生肠源性内毒素血症(IETM)时,肠黏膜屏障、肠型碱性磷酸酶(IAP)的变化及甘氨酸的保护机制。方法采用10%的果糖溶液喂养大鼠复制IETM模型,同时给予甘氨酸干预。分别于2月、4月末处死动物,检测血浆内毒素(LPS)及肠组织IAP水平;HE及Gomori染色检测肠肠黏膜屏障变化与IAP表达,分析LPS与IAP的相关性。结果与对照组相比,模型组2月、4月血浆LPS水平明显升高(P0.01),肠组织IAP表达明显降低(P0.01),肠黏膜屏障发生损伤;甘氨酸干预对上述变化有明显改善作用(P0.05);LPS与IAP水平负相关(rs=0.66,P0.05)。结论果糖饮食致大鼠肠黏膜屏障损伤是发生IETM的原因之一;甘氨酸通过促进肠IAP表达,减轻肠黏膜屏障损伤可明显改善IETM。
[Abstract]:Objective to investigate the intestinal mucosal barrier during the development of intestinal endotoxemia (IETM) induced by fructose diet in rats. The changes of intestinal alkaline phosphatase (IETM) and the protective mechanism of glycine. Methods the IETM model of rats was fed with fructose 10% and treated with glycine on February. At the end of April, the animals were killed and the plasma endotoxin levels and intestinal IAP levels were detected. The changes of intestinal mucosal barrier and the expression of IAP were detected by HE and Gomori staining. The correlation between LPS and IAP was analyzed. Results compared with the control group, the model group was February. In April, the level of plasma LPS increased significantly, the expression of IAP in intestinal tissue decreased significantly, and the intestinal mucosal barrier was damaged. Glycine intervention could significantly improve the above changes (P 0.05). There was a negative correlation between LPS and IAP level. Conclusion the injury of intestinal mucosal barrier induced by fructose diet is one of the causes of IETM in rats. Glycine can significantly improve the intestinal mucosal barrier injury by promoting the expression of IAP in the intestine.
【作者单位】：山西医科大学汾阳学院科技中心;
【基金】：山西省研究生优秀创新项目(No.20103052) 山西省高等学校大学生创新创业训练项目(No.2012394)
【分类号】：R151
【正文快照】： 肠黏膜屏障损伤致内毒素(endotoxin,LPS)吸收增加及肝脏枯否细胞吞噬功能下降是形成肠源性内毒素血症(Intestinal Endotoxemia,IETM)的主要原因。动物实验研究表明,IETM是促发多种代谢性疾病的始动因素之一[1,2]。本实验室前期研究发现,甘氨酸对果糖饮食所致的IETM有明显改善

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