不同污水中细菌多样性DGGE检测

发布时间：2018-01-26 01:35

本文关键词： 污染水源细菌多样性传统平板培养变性梯度凝胶电泳　出处：《中国公共卫生》2015年08期 　论文类型：期刊论文

【摘要】：目的比较工业、农业和生活污染源河道内细菌群落的多样性,探讨环境与细菌群落多样性关系,为修复水环境技术提供理论依据。方法采集浙江省杭州市未污染的虎跑山泉水和3个不同污染源的水样共70份,用传统微生物平板培养和变性梯度凝胶电泳(DGGE)技术分析不同水体细菌群落的多样性差异。结果未被污染的虎跑山泉水p H最小,为7.25,其余受到污染的水体p H均7.4,滨文河的p H最高,达8.04;浦沿化工厂河道中存在可培养的细菌最多,达到821 CFU/m L,其次为萧山养牛河道,虎跑最低仅8 CFU/m L,其细菌总数与p H密切相关;6种受污染的水中均存在大肠埃希菌和葡萄球菌,生活污水中未检测到沙门菌,化工污水中未检测到粪链球菌;变性梯度凝胶电泳结果表明,受污染的水中主要有变形菌门(23条带,71.9%)、放线菌门(8条带,25%)和厚壁菌门(1条带,3.9%),变形菌门是绝对的优势菌群;不同污染源影响的水中均有特异性细菌条带T5(uncultured bacterium clone C-84,HQ860591)和T7(uncultured rhodocyclaceae bacterium clone D7N55,KC006201),隶属于β-变形菌,只在受化工污染的河水中检测到;而受生活污水污染的河水中特有条带T3和T4均为γ-变形菌的气单胞菌属Aeromona;T10、T29和T30为受畜牧养殖场污水影响的河水中所特有,均隶属于放线菌门Gordonibacter属。结论不同的污染直接影响水的p H及细菌群落分布,且污水中致病菌也存在差异。
[Abstract]:Objective to compare the diversity of bacterial communities in the river channels of industrial, agricultural and domestic pollution sources, and to explore the relationship between the environment and the diversity of bacterial communities. In order to provide theoretical basis for the technology of water environment restoration, 70 samples of unpolluted Hu-run mountain spring water and three different pollution sources were collected from Hangzhou, Zhejiang Province. Traditional microbial plate culture and denaturing gradient gel electrophoresis (DGGE) technique were used to analyze the diversity of bacterial communities in different water bodies. The pH of the other polluted water bodies was 7.4, and the pH of Binwen River was the highest (8.04). The number of culturable bacteria was 821 CFU/m / L in the river channel of Puyan Chemical Plant, followed by Xiaoshan cattle channel, and the lowest was 8 CFU/m / L. The total number of bacteria was closely related to pH; Escherichia coli and Staphylococcus were found in 6 kinds of polluted water, no salmonella was detected in domestic sewage, no streptococcus faecalis was detected in chemical wastewater. The results of denaturing gradient gel electrophoresis showed that there were 23 bands of Proteus in polluted water, 8 bands of actinomycetes (25%) and 1 band of phylum (3. 9). The phylum of Proteus is an absolute dominant flora. The specific bacterial band T5unculated bacterium clone C-84 was found in the water affected by different pollution sources. HQ860591) and T7unculated rhodocyclaceae bacterium clone D7N55. KC006201, belonging to 尾 -Proteus, was only detected in the river polluted by chemical industry. Aeromonas aeromonas, where the endemic bands T3 and T4 are 纬 -Proteus, belong to Aeromonas. T10, T29 and T30 are endemic to rivers affected by sewage from livestock farms. All belong to Gordonibacter genus of actinomycetes. Conclusion different pollution directly affects the distribution of pH and bacterial community in water, and there are also differences in pathogenic bacteria in sewage.
【作者单位】：浙江中医药大学生命科学学院生物技术研究所;
【基金】：浙江省高校中青年学科带头人学术攀登项目(pd2013215) 浙江省大学生科技创新活动计划暨新苗人才计划(2013R410030)
【分类号】：R123
【正文快照】： 中国目前水污染情况严重,据预测,2015年废水排放量将达到810亿吨[1]。目前水污染主要包括工业污染源、农业污染源和生活污染源。工业污染源为水域的重要污染源,它具有量大、面广、成分复杂、毒性大、不易净化、难处理等特点;农业污染源包括牲畜粪便、农药、化肥等。生活污染源

【参考文献】