枸杞多糖及其配伍茶多酚缓解小鼠体力疲劳作用的研究

发布时间：2018-02-09 05:44

本文关键词： 枸杞多糖茶多酚配伍体力疲劳　出处：《上海交通大学学报(医学版)》2016年05期 　论文类型：期刊论文

【摘要】：目的评价枸杞多糖(LBP)及LBP配伍茶多酚(TP)缓解小鼠体力疲劳作用的效果,并探讨其作用机制。方法 SPF级雄性昆明小鼠336只,共分为4批,每批随机分成7组:LBP低、中、高剂量(LBP-L、LBP-M、LBP-H)组,LBP配伍TP(以下简称配伍组)低、中、高剂量(C-L、C-M、C-H)组,设生理盐水(NS)为对照,每组12只。小鼠在每日饲喂基础饲料的基础上,以每10 g体质量0.2 m L溶液灌胃,15 d后进行负重游泳实验,并检测肝糖原、血清尿素氮和血乳酸等生化指标。结果同NS组相比,LBP-M、LBP-H组小鼠负重游泳时间显著延长(P0.05),LBP-L、LBP-M、LBP-H组肝糖原的含量显著增加(P0.05),血清尿素氮和血乳酸水平显著降低(P0.05);C-L、C-M、C-H组小鼠负重游泳时间显著延长(P0.05),肝糖原的含量显著提高(P0.05),血清尿素氮和血乳酸水平显著降低(P0.05)。同LBP组相比,配伍组负重游泳时间延长,肝糖原含量增加,血尿素氮和血乳酸水平降低;其中C-L组肝糖原水平显著高于LBP-L组(P=0.027),C-M组血乳酸水平显著低于LBP-M组(P=0.006)。结论 LBP及LBP配伍TP对小鼠体质量无影响,但具有缓解小鼠体力疲劳的作用;其作用机制可能与其增加了肝糖原储备量、减少蛋白质的分解代谢,进而减少血清尿素氮水平及增加了磷酸果糖激酶的活性,使血乳酸水平下降有关。
[Abstract]:Objective to evaluate the effect of Lycium barbarum polysaccharides (LBP) and LBP combined with tea polyphenols (LBP) on relieving physical fatigue in mice, and to explore its mechanism. Methods 336 SPF grade male Kunming mice were divided into 4 batches and randomly divided into 7 groups. The high dose LBP-LBP-MU LBP-H group was low in LBP and TP- (hereinafter referred to as the compatibility group). The control group consisted of 12 mice in each group, and 12 mice in each group were fed with basic diet daily. The weight bearing swimming experiment was carried out 15 days later with 0.2 mL solution per 10 g body mass, and liver glycogen was detected. Results compared with NS group, the swimming time of LBP-MU LBP-H group was prolonged significantly, the content of liver glycogen in LBP-LBP-LBP-H group increased significantly, and the level of serum urea nitrogen and blood lactic acid decreased significantly. The swimming time was prolonged significantly (P 0.05), liver glycogen content was significantly increased, serum urea nitrogen and blood lactic acid levels were significantly decreased compared with LBP group. In the compatibility group, the swimming time was prolonged, the content of liver glycogen was increased, and the levels of blood urea nitrogen and blood lactic acid were decreased. The level of liver glycogen in C-L group was significantly higher than that in LBP-L group. The serum lactate level in C-M group was significantly lower than that in LBP-M group. Conclusion LBP and LBP combined with TP have no effect on the body weight of mice, but they can relieve physical fatigue of mice. The mechanism may be related to the increase of liver glycogen reserve, the reduction of catabolism of protein, the decrease of serum urea nitrogen level and the increase of phosphofructokinase activity, and the decrease of blood lactate level.
【作者单位】：上海交通大学公共卫生学院;
【分类号】：R151

【相似文献】