海参活性成分对高尿酸血症的影响

发布时间：2018-02-11 23:38

本文关键词： 海参海参皂苷海参多糖高尿酸血症　出处：《中国海洋大学》2012年硕士论文　论文类型：学位论文

【摘要】：海参是重要的食物和药物资源，含有多种活性物质，具有抗肿瘤、抗真菌、抗氧化，免疫调节、降血脂等方面的作用。本实验，研究了海参及其活性成分海参多糖(sea cucumber polysaccharides, PSC)、海参皂苷(sea cucumber saponin,SSC)对饮食诱导以及肥胖伴随的高尿酸血症小鼠尿酸代谢的影响，并对其作用机制进行初步探讨，主要内容包括以下几部分： 1．喂食小鼠酵母浸粉建立饮食诱导的高尿酸血症小鼠模型，研究了海参皂苷和除皂苷残余物对高尿酸血症的影响，结果发现两者均具有良好的改善酵母浸粉致高尿酸血症的作用。喂养14天后，海参皂苷低、高剂量组血清尿酸(Uric Acid，UA)水平分别较对照组降低53.1%(P 0.01)与55.6%(P 0.01)，除皂苷残余物组在第9天时可显著降低尿酸水平，但在14天时无显著性差异。进一步研究发现，海参皂苷及其除皂苷残余物抑制小鼠血清尿酸升高与肝脏尿酸合成代谢关键酶黄嘌呤氧化酶(Xanthine Oxidase，XOD)和腺苷脱氨酶(Adenosine Deaminase，ADA)的活性受到抑制明显相关，该实验结果同时证明，海参中含有的抑制血中尿酸水平升高的活性物质除了皂苷外，还有其他活性成分存在。 2．根据上述实验结果，本研究从海参除皂苷残余物中提取了海参多糖，进一步比较研究了整海参、海参皂苷、海参多糖对酵母浸粉诱导的高尿酸血症小鼠的尿酸代谢的影响，，并同时比较了菲律宾刺参(Pearsonothuria graeffei，Pg)、仿刺参(Apostichopus japonicus，Aj)、冰岛刺参(Cucumaria frondosa，Cf)和美国肉参(Isostichopus badionotus，Ib)对高尿酸血症的影响的差异，并从基因转录调节水平探讨其作用机理。结果表明，海参皂苷和海参多糖以及四种海参均对酵母浸粉诱导的高尿酸血症有明显的改善作用。喂养14天后，各组尿酸水平均显著下降，下降幅度分别是皂苷组14.8%(P 0.05)；多糖组20.9%(P 0.01)；菲律宾刺参组16.1%(P 0.05)、仿刺参组20.1%(P 0.01)、冰岛刺参组12.2%(P 0.05)和美国肉参组25.3%(P 0.01)。各组均可以显著抑制肝脏XOD和ADA的活性，同时海参皂苷和海参多糖还可以下降XOD和ADA mRNA表达，下调肾脏葡萄糖转运体9(GLUT9) mRNA表达，抑制尿酸的重吸收，上调肾脏有机阳离子和肉毒碱转运体(OCTs和OCTNs) mRNA表达，改善肾功能。 3．根据上诉实验结果，本文进一步研究了海参皂苷单体EA对肥胖伴随的高尿酸血症小鼠(db/db小鼠)的尿酸代谢和肾功能的影响，并通过基因转录调节水平和显微结构观察探讨其机理。结果发现，海参皂苷单体EA可显著降低db/db小鼠的血清尿酸水平，分别降低了18.1%(P 0.05)和12.7%(P 0.05)，但并不降低肝脏XOD和ADA的活性和mRNA表达。EA可上调肾脏有机阴离子转运体1(OAT1)mRNA表达，促进尿酸的排泄，上调肾脏OCTs和OCTNs的mRNA表达，改善肾功能。光镜和电镜的观察结果也显示EA可恢复肾脏结构，保护肾脏，这与基因转录调节水平得到的结论相一致。综上所述，本文研究了海参及海参活性成分对饮食诱导的高尿酸血症和肥胖伴随的高尿酸血症动物尿酸代谢的影响，并初步探讨了其作用机制，为海参的综合利用提供了科学依据。
[Abstract]:The sea cucumber is an important food and drug resource, contains a variety of active substances with antitumor, antifungal, antioxidant, immunomodulatory, lowering lipid. In this experiment, the sea cucumber and its active constituents of sea cucumber polysaccharide (sea cucumber polysaccharides PSC (sea cucumber), holothurin saponin, SSC) on diet and the effect of induced hyperuricemia mice uric acid metabolism in obesity, and to explore its mechanism, the main contents include the following parts:
1. mice fed yeast extract to establish hyperuricemia mouse model of diet induced, studied and effects of saponins of sea cucumber saponin in residue of hyperuricemia, found that both has good improvement of yeast extract powder induced hyperuricemia effect. 14 days later, holothurin low, high dose group of serum uric acid (Uric Acid, UA) level compared with the control group decreased 53.1% (P 0.01) and 55.6% (P 0.01), in addition to saponin residue group can significantly reduce the level of uric acid in ninth days, but on the 14 day there was no significant difference. Further studies showed that the sea cucumber saponins and its saponin residue in mice with elevated serum uric acid inhibition of key enzyme of liver xanthine and uric acid anabolic enzymes (Xanthine Oxidase, XOD) and adenosine deaminase (Adenosine Deaminase, ADA) significantly inhibited the activity of the related experimental results also proved that the inhibition of blood contained in sea cucumber In addition to the saponins, the active substance in the middle uric acid level also exists in other active components.
2. according to the above results, this study from the sea cucumber saponins except residue extracted from sea cucumber polysaccharide, further comparative study of the effects of saponins of sea cucumber, sea cucumber, sea cucumber polysaccharide on uric acid metabolism in yeast extract induced hyperuricemia mice, and compared the Philippines sea cucumber (Pearsonothuria graeffei, Pg), sea cucumber (Apostichopus japonicus, Aj), sea cucumber (Cucumaria frondosa, Cf Iceland) and American ginseng (Isostichopus badionotus, Ib meat) influence on hyperuricemia, and from the gene transcription level to explore its mechanism. The results showed that the sea cucumber saponin and polysaccharide and four kinds of sea cucumber on yeast extract induced with hyperuricemia obvious improvement. 14 days later, the level of uric acid levels were significantly decreased, decreased saponin group 14.8% (P 0.05); polysaccharide group 20.9% (P 0.01); Philippines sea group 1 6.1% (P 0.05), Apostichopus japonicus group 20.1% (P 0.01), Iceland sea cucumber group 12.2% (P 0.05) and American ginseng meat group 25.3% (P 0.01). Each group can significantly inhibit the liver XOD and ADA activity, while the sea cucumber saponin and polysaccharides can also decrease XOD and ADA expression of mRNA and down-regulation of renal the dirty glucose transporter 9 (GLUT9) mRNA expression, inhibiting uric acid reabsorption and increased renal organic cation and carnitine transporters (OCTs and OCTNs) mRNA expression, improve renal function.
3. according to the experimental results, this paper further studies the sea cucumber saponin monomer EA on obesity with hyperuricemia mice (db/db mice) influence the uric acid metabolism and renal function, and through the regulation of gene transcription level and explore the mechanism of microstructure observation. The results showed that the sea cucumber saponin monomer EA can significantly decrease the level of serum uric acid in db/db mice the decreased 18.1% (P 0.05) and 12.7% (P 0.05), but did not reduce the liver XOD and ADA activity and mRNA expression of.EA can increase the renal organic anion transporter 1 (OAT1) mRNA expression, promote the excretion of uric acid, the upregulation of the expression of kidney OCTs and OCTNs mRNA, improve renal function. Results the observation of light and electron microscopy also showed that EA can restore the renal structure, protect the kidney, which is consistent with the level of gene transcription regulation conclusion.
In conclusion, the effects of sea cucumber and sea cucumber active ingredients on uric acid metabolism in hyperuricemia and obesity accompanied by hyperuricemia in animals were studied, and its mechanism was preliminarily discussed, which provided a scientific basis for the comprehensive utilization of sea cucumber.

【学位授予单位】：中国海洋大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：TS254.1;R151

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