山楂黄烷醇延缓衰老活性及其作用机制研究

发布时间：2018-02-20 01:10

本文关键词： 山楂黄烷醇衰老 D-半乳糖抗氧化酶脂质过氧化物　出处：《河北农业大学》2013年硕士论文　论文类型：学位论文

【摘要】：人口老龄化是我国等多个国家都在面临的严峻问题，因而衰老及其机理研究受到了广泛的重视。本文依托衰老的自由基学说、通过体内试验对山楂黄烷醇（MF）的延缓衰老活性和作用机制进行了探索，为进一步开发保健食品新资源提供依据。研究方法：（1）MF延缓果蝇衰老活性研究。利用果蝇生存实验模型观察受试物对雌、雄果蝇寿命参数——平均寿命、最高寿命和累计死亡曲线的影响。并对饲养30d的雌、雄果蝇进行生化指标检测，评估受试物在果蝇体内的抗氧化活性。（2）MF对D-半乳糖诱导小鼠过氧化损伤的拮抗作用。在对小鼠皮下注射D-半乳糖以制造过氧化损伤模型的过程中，同时灌胃给予受试物，连续49d；以小鼠血清和脏器的抗氧化酶（SOD、GSH-Px）活性、脂质过氧化产物（MDA）含量及肝、脑衰老指示酶（MAO）活性为检测指标，评估受试物对D-半乳糖诱导小鼠过氧化损伤的保护作用和预防衰老作用。（3）MF对衰老模型小鼠机体抗氧化活性的影响。首先通过皮下注射D-半乳糖的方式复制衰老小鼠模型，造模成功后，灌胃给予受试物，连续35d；以衰老小鼠血清和脏器的抗氧化酶（SOD、GSH-Px）活性、脂质过氧化产物（MDA）含量及肝、脑衰老指示酶（MAO）活性、脑ATP酶（Na+K+ATPase）为检测指标，考察受试物对老龄动物机体抗氧化水平的改善和恢复作用。研究结果：（1）MF延缓果蝇衰老活性。MF可以提高雌、雄果蝇平均寿命和最高寿命，并推迟半数死亡时间；0.2%和0.8%剂量组具有显著的延寿作用，且整体而言对雌果蝇效果更好；上述两剂量组对雌果蝇的平均寿命延长率分别为6.6%和7.2%，最高寿命延长率分别为5.8%和12.1%，对雄果蝇的平均寿命延长率分别5.3%和5.8%，最高寿命延长率分别为和11.7%和10.2%。MF也可提升果蝇体内SOD活力和降低MDA水平，0.2%剂量组效果最好，，对雌果蝇效果更好。MF的延寿作用可能与其抗氧化活性有关。（2）MF对D-半乳糖诱导小鼠过氧化损伤的拮抗作用。在造模的同时给予MF可以拮抗D-半乳糖诱导的小鼠体内过氧化损伤过程，不同剂量MF及阳性对照物（100mg/kg.bw维生素C）在不同样本中活性不同。从抗氧化指标和衰老指示酶分析，60mg/kg.bw的MF有局部的抗氧化和预防衰老活性，120mg/kg.bw的MF则有较全面的抗氧化和延缓衰老活性，60~120mg/kg.bw对主要脏器中MDA的清除效果要优于阳性对照，而阳性对照组对血清各指标的效果较好，其它指标MF与阳性对照组各有千秋。（3）MF对衰老模型小鼠机体抗氧化活性的影响。研究表明，不同剂量的MF可不同程度的减轻衰老模型小鼠的老化状态、改善其抗氧化水平。90~180mg/kg.bw的MF在衰老动物血清和脏器内具有抗氧化活性，并可提升脑Na+K+ATPase水平，且高剂量效果更好、更全面；60mg/kg.bwMF可降低脑MAO活性，90~180mg/kg.bwMF可降低肝MAO活性。MF抗衰老作用的最佳作用条件还有待进一步探索。
[Abstract]:In this paper , the mechanism of aging and its mechanism of aging and its mechanism are studied in this paper . Based on the theory of free radical in aging , the mechanism of delaying aging and the mechanism of action of hawthorn flavanol ( MF ) is explored through in vivo experiments , which provides the basis for further development of new resources for health food . Study method : ( 1 ) The effects of the test article on the life parameters , mean life , maximum life and cumulative death curve of female and male fruit fly were observed by using the survival experiment model of fruit fly . ( 2 ) The effect of MF on the peroxidation damage induced by D - galactose in mice was studied . In the process of producing peroxide injury model by subcutaneous injection of D - galactose , the activity of antioxidant enzymes ( SOD , GSH - Px ) , lipid peroxidation products ( MDA ) and liver and brain aging indicator enzyme ( MAO ) were measured . ( 3 ) The effects of MF on anti - oxidation activity of aging model mice were studied . First , the aging mice model was duplicated by subcutaneous injection of D - galactose . After successful molding , the test article was injected into the stomach for 35 days . The activities of antioxidant enzymes ( SOD , GSH - Px ) , lipid peroxidation products ( MDA ) and liver , brain aging indicator enzyme ( MAO ) activity and brain ATP enzyme ( Na + K + ATPase ) were measured by subcutaneous injection of D - galactose . Results of the study : The results showed that MF could increase the average life expectancy and the maximum life of the female and male fruit fly , postpone the half dead time ; 0.2 % and 0.8 % of the dose groups had significant life prolonging effects . The average life extension rate of the two groups were 6 . 6 % and 7 . 2 % respectively . ( 2 ) The effect of MF on peroxidation damage induced by D - galactose in mice induced by D - galactose . MF and positive control ( 100mg / kg . bw vitamin C ) had different activity in different samples . ( 3 ) The effect of MF on anti - oxidation activity of aging model mice was studied .

【学位授予单位】：河北农业大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R151.2

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