兔煤尘肺各期高分辨率CT表现与肺巨噬细胞含量的相关研究

发布时间：2018-02-20 07:52

本文关键词： 兔煤尘肺巨噬细胞肺纤维化 HRCT　出处：《山西医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：通过动态观察兔煤尘肺病程过程，探索兔煤尘肺各期AM（肺泡巨噬细胞）和IM（间质巨噬细胞）含量的变化趋势与肺纤维化进展的关系。方法： 1、实验动物采用40只健康雄性日本大耳白兔，，随机分为5组，每组任选5只作为实验对象煤尘组，剩余每组另外3只作为对照组，麻醉状态下通过新生儿喉镜引导分别对煤尘组兔气管内一次性注入100mg/ml的煤尘悬液（灌注量2ml/kg)；对照组采用同样的方法注入同等剂量的生理盐水。 2、分别于2周、1月、2月、3月、5月时对相应的各组兔子进行HRCT扫描分析，通过对实验兔子肺的影像学表现来判断其肺纤维化进程。 3、每一期实验兔子扫描后处死，取出肺组织，用机械法分离AM（肺泡巨噬细胞），用酶消化法分离IM（间质巨噬细胞），并用流式细胞仪对其含量定量分析并与HRCT改变对照。 4、实验数据经SPSS13.0统计软件采用秩和检验和Spearman秩相关分析进行统计，以α=0.05作为检验水准，P＜0.05差异有统计学意义。结果：煤尘组兔HRCT动态观察发现肺病变明显的进展，2周-1月HRCT显示肺内主要出现支气管血管束异常、磨玻璃样密度影、小叶间隔增厚及结节影，3月-5月HRCT显示煤尘肺主要出现异常扩张的支气管、肺蜂窝状改变及肺不张等HRCT表现，流式细胞仪测定AM（肺泡巨噬细胞）及IM（间质巨噬细胞）全部观察各个时间点，所有实验组统计结果显示：煤尘组各个时间点的AM%差别有统计学意义(H=23.077，P0.001)，对照组各个时期的AM%差别没有统计学意义（H=1.885，P=0.757）。煤尘组各个时间点的IM%差别有统计学意义(H=22.607，P0.001)，对照组各个时期的IM%差别没有统计学意义（H=3.290，P=0.511）。AM%与HRCT严重程度的相关系数rs=0.746；IM%与HRCT严重程度的相关系数rs=-0.776；AM%与IM%的相关系数rs=-0.968。HRCT表现与各期AM（肺泡巨噬细胞）及IM（间质巨噬细胞）含量均具有相关性；生理盐水对照组各期无上述改变。结论： HRCT能动态反应各期兔煤尘肺的影像学变化，各期病程在HRCT上可观察到；兔煤尘肺模型的各期HRCT表现和AM（肺泡巨噬细胞）及IM（间质巨噬细胞）含量存在相关性。AM（肺泡巨噬细胞）及IM（间质巨噬细胞）含量之间存在相关性，从而证明肺巨噬细胞含量与HRCT表现具有较大相关性。
[Abstract]:Objective:. Through dynamic observation of the process of coal pneumoconiosis in rabbits, the relationship between the changes of AM (alveolar macrophage) and IMM (interstitial macrophage) and the progression of pulmonary fibrosis in each stage of rabbit coal pneumoconiosis was investigated. Methods:. 1. Forty healthy male Japanese white rabbits were randomly divided into 5 groups, 5 rabbits in each group as experimental objects, and 3 rabbits in each group as control group. Under anesthesia, 100 mg / ml coal dust suspension was injected into the trachea of the coal dust group by laryngoscope respectively (2 ml / kg / kg) and the control group was injected with the same dose of normal saline by the same method. 2. Two weeks, January, February, March, March, respectively, HRCT scanning analysis was performed on each group of rabbits, and the pulmonary fibrosis progression was judged by imaging findings of the lungs of experimental rabbits. 3. The rabbits were killed after each phase of scanning, and the lung tissue was taken out. The alveolar macrophages were separated by mechanical method, and the interstitial macrophages were separated by enzyme digestion. The contents of the macrophages were quantitatively analyzed by flow cytometry and compared with the changes of HRCT. 4. The experimental data were statistically analyzed by SPSS13.0 software using rank sum test and Spearman rank correlation analysis. There was significant difference in the test level between 伪 -0. 05 and Spearman (P < 0. 05). Results:. The dynamic observation of HRCT in the coal dust group revealed the obvious progress of pulmonary lesions from 2 weeks to January HRCT showed the main abnormalities of bronchovascular bundle in the lung, and the ground-glass-like density shadow. The interlobular septal thickening and nodular shadow were observed. From March to May, HRCT showed that coal pneumoconiosis mainly showed abnormal dilation of bronchi, honeycomb changes of lung, atelectasis and other HRCT manifestations. Flow cytometry was used to measure AM (alveolar macrophage) and IMM (interstitial macrophage) at all time points. The results of all experimental groups showed that the difference of AM% at different time points in coal dust group was statistically significant, while that in control group was not significant at every stage. The difference of IM% in coal dust group at different time points was statistically significant. There was no significant difference in IM% between different periods of exposure group. The correlation coefficient between HRCT severity and HRCT severity was not statistically significant. The correlation coefficient between IM% and HRCT severity was r sl0. 776% and IM%. Rs=-0.968.HRCT expression and the expression of AM (alveolar macrophage) and IMM (mesenchymal macrophage) in all stages were not statistically significant. There was a correlation between the cell content and the cell content. No above changes were found in the saline control group. Conclusion:. HRCT could dynamically reflect the imaging changes of rabbit coal pneumoconiosis at different stages, and the course of disease in each stage could be observed on HRCT. There was a correlation between HRCT findings and the contents of AM (alveolar macrophage) and IMM (interstitial macrophage) in each stage of rabbit coal pneumoconiosis model. There was a correlation between the contents of AM (alveolar macrophage) and IMM (interstitial macrophage). It was proved that the content of pulmonary macrophages was correlated with HRCT.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R135.2;R816.41

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