枸杞汁对DEHP致大鼠氧化损伤和血脂异常保护作用研究

发布时间：2018-02-20 19:54

本文关键词： DEHP 枸杞汁谷胱甘肽氧化损伤血脂异常　出处：《毒理学杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨枸杞汁对邻苯二甲酸二乙基己基酯(DEHP)染毒所致氧化损伤和血脂异常的干预作用及差异。方法将实验大鼠分为对照组、DEHP组(1 500 mg/kg)、GSH干预组(500 mg/kg GSH+1 500 mg/kg DEHP)和枸杞汁干预组(枸杞原汁+1 500 mg/kg DEHP),连续灌胃7 d,测定大鼠血清及肝中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性以及血脂水平。结果与对照组相比,DEHP染毒会导致血清中GSH-Px活性、甘油三酯(TG)、高密度脂蛋白(HDL-C)浓度降低,差异有统计学意义(P0.05或P0.01),TG/HDL-C和非高密度脂蛋白胆固醇(non-HDL-C)浓度升高,差异有统计学意义(P0.05或P0.01),肝中GSH-Px、SOD活性差异有统计学意义(P0.05或P0.01);与DEHP组比较,枸杞汁和GSH均能使血清中GSH-Px活性升高,TG/HDL-C降低(P0.05或P0.01),枸杞汁组non-HDL-C、GSH组LDL-C浓度降低(P0.05),2个干预组大鼠肝中SOD活性均低于DEHP组,上述差异均有统计学意义。结论 DEHP染毒会导致大鼠肝氧化损伤及血脂水平异常,枸杞汁与GSH的作用相似,即对DEHP诱导的氧化损伤和血脂异常具有良好的调节保护作用。
[Abstract]:Objective to investigate the effects of Lycium barbarum juice on oxidative damage and dyslipidemia induced by diethylhexyl phthalate (DEHP). Methods the experimental rats were divided into control group (1 500 mg / kg) and control group (n = 1 500 mg / kg). The activity of glutathione peroxidase (GSH-PxX) and superoxide dismutase (SOD) in serum and liver of rats were measured in the medlar juice intervention group (medlar juice 1 500 mg/kg DEHPX) for 7 days. Results compared with the control group, the rats were exposed to DEHP. Lead to GSH-Px activity in the serum, The concentration of HDL-C in TGN and HDL-C decreased, the difference was statistically significant (P0.05 or P0.01 / TG / HDL-C and non-HDL-C), and the concentration of HDL-C was significantly higher than that of non-HDL-C. The difference was statistically significant (P 0.05 or P 0.01), and the activity of SOD in liver was significantly higher than that in DEHP group (P 0.05 or P 0.01). Both Lycium barbarum juice and GSH could increase the activity of TG- / HDL-C in serum and decrease the activity of TG- / HDL-C by P0.05 or P0.01. The concentration of LDL-C in non-HDL-CG-GSH group in Lycium barbarum juice group decreased P0.05A, and the activity of SOD in liver of the two intervention groups was lower than that of DEHP group. Conclusion DEHP exposure can lead to liver oxidative injury and abnormal blood lipids in rats. The effect of Lycium barbarum juice is similar to that of GSH, that is, it has a good regulatory and protective effect on DEHP induced oxidative damage and dyslipidemia.
【作者单位】：华南农业大学食品学院广东省食品质量安全重点实验室;青海大漠红枸杞有限公司;广东产品质量监督检验研究院;
【基金】：农业部农产品质量安全监管项目(GJFP201501202) 广东省自然科学基金(2016A030313395) 广东省现代农业产业技术体系创新团队建设项目(2016LM2152)
【分类号】：R114

【相似文献】