2013年中国桶装水铜绿假单胞菌的复核验证及耐药特征分析

发布时间：2018-03-04 04:10

本文选题：铜绿假单胞菌　切入点：PCR　出处：《中国食品卫生杂志》2016年02期 　论文类型：期刊论文

【摘要】：目的了解2013年中国21个省市自治区桶装水来源铜绿假单胞菌的鉴定正确率,对我国桶装水来源铜绿假单胞菌的耐药状况进行初步分析。方法采用基于eta2和opr I两对基因的PCR方法对桶装水来源铜绿假单胞菌进行快速复核,使用Vitek GN生化鉴定卡进行生化验证,评价分离菌株的复核正确率和PCR方法的准确度,采用微量肉汤稀释法,对8类12种抗生素的耐药性进行测定。结果两对引物均能对铜绿假单胞菌扩增出预期的目的条带,但eta2具有更高的特异性;抽样进行生化试验结果显示2013年各地上报并运送的菌株复核正确率为100%;两种基因的PCR方法准确度均在95%以上,但均存在较低比例假阴性;2013年中国21个省市自治区上报的531株铜绿假单胞菌耐药率为11.68%(62/531),主要耐受多粘菌素B(5.27%,28/531)、氨曲南(4.14%,22/531)和美罗培南(3.01%,16/531),替卡西林/克拉维酸和替卡西林的中介率分别为43.31%(230/531)和25.42%(135/531)。结论各地上报分离的铜绿假单胞菌的准确性较高,PCR方法可对桶装水来源铜绿假单胞菌进行快速筛选,结合生化鉴定方法可准确鉴定。桶装水来源铜绿假单胞菌的耐药处于较低水平,但替卡西林/克拉维酸和替卡西林某种程度上显示了一定的耐药趋势,需要定期监测,以阐明桶装水中的铜绿假单胞菌的耐药特征和耐药趋势。
[Abstract]:Objective to investigate the accuracy of identification of Pseudomonas aeruginosa from bottled water in 21 provinces and autonomous regions of China in 2013. The drug resistance of Pseudomonas aeruginosa from barrelled water in China was preliminarily analyzed. Methods the PCR method based on eta2 and opr I genes was used for rapid review of Pseudomonas aeruginosa from barrelled water. The Vitek GN biochemical identification card was used for biochemical verification, and the accuracy of the PCR method and the rechecking accuracy of the isolated strains were evaluated, and the trace broth dilution method was used to evaluate the accuracy of the method. Results two pairs of primers could amplify the desired target bands of Pseudomonas aeruginosa, but eta2 had higher specificity. The results of biochemical tests showed that in 2013, the correct check rate of the strains reported and transported was 100%, and the accuracy of PCR method for both genes was more than 95%. In 2013, the drug resistance rate of 531 Pseudomonas aeruginosa strains reported in 21 provinces and autonomous regions of China was 11.68 / 532 / 531, mainly tolerant of polymyxin BU 5.2728 / 531, amtreonam 4.1422 / 531) and meropenem 3.01 / 16531, tikacillin / clavulanic acid and tekassie. The mediating rates of the forests were 43.31% and 25.42%, respectively. Conclusion the accuracy of the isolated Pseudomonas aeruginosa reported in different regions is higher. PCR can be used to screen the Pseudomonas aeruginosa from bottled water source quickly. The drug resistance of Pseudomonas aeruginosa from barreled water was at a low level, but the resistance of ticacillin / clavulanic acid and ticacillin to some extent showed a certain trend of drug resistance, which needed to be monitored regularly. To elucidate the drug resistance characteristics and drug resistance trend of Pseudomonas aeruginosa in barrelled water.
【作者单位】：国家食品安全风险评估中心卫生部食品安全风险评估重点实验室;山东大学公共卫生学院;
【基金】：863计划“动植物源食品中内源有害物精准检测技术”课题资助(2012AA101603)
【分类号】：R155

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