橄榄油对新生期双酚A暴露后青春期雄性小鼠生殖毒性的拮抗作用

发布时间：2018-03-04 13:18

本文选题：双酚A　切入点：橄榄油　出处：《环境与健康杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的研究橄榄油对新生期双酚A(bisphenol A,BPA)暴露后青春期雄性小鼠生殖毒性的拮抗作用。方法将32只清洁级新生ICR雄性小鼠随机分为4组,分别为对照(玉米油)组、橄榄油组、BPA(100μg/kg,玉米油稀释,前期实验中能明显引起精母细胞减数分裂阻滞)组、橄榄油+BPA(100μg/kg)组,每组8只。自出生第一天(PND 1)至PND 21(断乳),仔鼠每天进行颈背部皮下注射染毒,染毒容量为4μl/g,每天1次,连续染毒至断乳(PND 21)。PND 30,测定睾丸曲细精管直径、生精上皮厚度、管腔直径以及睾丸组织中Boule mRNA和蛋白的表达水平。结果与对照组相比,BPA组小鼠睾丸曲细精管直径和生精上皮厚度均显著下降,曲细精管管腔直径显著上升,差异均有统计学意义(P0.05);虽然橄榄油+BPA组与对照组相比仔鼠曲细精管直径和生精上皮厚度也有明显下降(P0.05),但与BPA组相比,曲细精管直径和生精上皮厚度均显著上升(P0.05),曲细精管管腔直径显著下降(P0.05)。与对照组相比,BPA组小鼠睾丸组织中Boule蛋白的表达下降,差异均有统计学意义(P0.05)。橄榄油+BPA组小鼠睾丸组织中Boule蛋白的表达水平高于BPA组,差异有统计学意义(P0.05)。与对照组相比,BPA组和橄榄油+BPA组小鼠睾丸组织中Boule mRNA的表达水平均降低,差异有统计学意义(P0.05)。但橄榄油+BPA组小鼠睾丸组织中Boule mRNA的表达水平高于BPA组,差异有统计学意义(P0.05)。结论橄榄油对BPA所致雄性小鼠生殖毒性及Boule表达下降有一定的拮抗作用。
[Abstract]:Objective to study the antagonistic effect of olive oil on reproductive toxicity of adolescent male mice exposed to bisphenol A bisphenol BPA.Methods Thirty-two freshmen ICR mice of clean grade were randomly divided into 4 groups: control group (corn oil). Olive oil group (100 渭 g 路kg ~ (-1)), corn oil was diluted, and in previous experiment, spermatocyte meiosis block was obviously induced. Olive oil BPA(100 渭 g / kg group, 8 rats in each group, from the first day of birth (PND1) to PND 21 (weaning milk), the young mice were injected subcutaneously into the neck and back every day. The dosing capacity was 4 渭 l / g, once a day, and it was continuously administered to PND 21. PND30. The diameter of testicular seminiferous tubules and the thickness of seminiferous epithelium were measured. Results compared with the control group, the diameter of seminiferous tubules and the thickness of seminiferous epithelium decreased significantly, and the diameter of seminiferous tubule increased significantly. Although the diameter of seminiferous tubules and the thickness of seminiferous epithelium decreased significantly in the BPA group compared with the control group, the difference was significant compared with the BPA group. The diameter of seminiferous tubules and the thickness of seminiferous epithelium increased significantly, while the diameter of seminiferous tubules decreased significantly. Compared with the control group, the expression of Boule protein in testis of BPA-treated mice decreased significantly. The expression level of Boule protein in testis of BPA group was higher than that of BPA group. Compared with the control group, the expression of Boule mRNA in testis of BPA group and olive oil BPA group decreased, and the difference was statistically significant (P 0.05). However, the expression level of Boule mRNA in testis of BPA group was higher than that of BPA group. Conclusion Olive oil has antagonistic effect on reproductive toxicity and Boule expression of male mice induced by BPA.
【作者单位】：潍坊医学院生物科学与技术学院;潍坊医学院医学研究实验中心;
【基金】：山东省自然科学基金(ZR2011CL015) 山东省医药卫生科技发展计划(2016WS0671) 地方高校国家级大学生创新创业训练计划(201510438003)
【分类号】：R114

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