矽尘对小鼠肺组织TLR4 mRNA与RelA mRNA表达的影响

发布时间：2018-03-05 04:27

本文选题：矽尘　切入点：TLR　出处：《环境与职业医学》2017年01期 　论文类型：期刊论文

【摘要】：[目的]探讨矽尘对小鼠肺组织TOLL样受体4(TLR4)mRNA与核因子-κB p65(RelA)mRNA表达的影响。[方法]将40只小鼠随机分成4组,每组10只,分别为2、4、8 h暴露组(即每天吸入染尘2、4、8 h)和对照组(不染尘)。染尘20 d后,取肺组织,应用实时荧光定量PCR法(RT-q PCR)测定各组TLR4 mRNA和RelA mRNA的表达。[结果]对照组与3个暴露组TLR4 mRNA表达量的中位数分别为0.89、1.22、4.89、7.21,4个组间的差异有统计学意义(χ~2=4.93,P=0.02);4、8 h暴露组与对照组的差异有统计学意义(P0.05),2 h暴露组与对照组的差异无统计学意义(P0.05)。对照组和3个暴露组RelA mR NA表达量分别为0.96、1.33、2.23、3.04,4个组间差异有统计学意义(χ~2=6.35,P=0.00);4、8 h暴露组与对照组的差异有统计学意义(P0.05),2 h暴露组与对照组的差异无统计学意义(P0.05)。TLR4 mRNA与RelA mRNA两者之间具有相关性(r=0.99,P=0.00)。[结论]矽尘染毒后小鼠肺组织TLR4 mRNA和RelA mRNA的表达上调,提示TLR4/RelA信号通路激活。
[Abstract]:[objective] to investigate the effects of silica dust on the expression of TOLL like receptor 4TLR4N mRNA and nuclear factor- 魏 B p65tir RelA mRNA in lung tissue of mice. [methods] 40 mice were randomly divided into 4 groups, 10 in each group. The lung tissues were taken out after 20 days of exposure to dust for 20 days, respectively, in the exposure group (2: 4) and in the control group (20 days after exposure to dust 2: 4) and the control group (20 days after exposure to dust) and the control group (n = 20), respectively. The expression of TLR4 mRNA and RelA mRNA was detected by real-time fluorescence quantitative PCR assay. [results] the median of TLR4 mRNA expression in the control group and the three exposed groups was 0.89 ~ 1.22 ~ 4.89 ~ 7.21, respectively. There was a significant difference among the four groups (蠂 ~ (2 +) 4.93) and the control group for 48 h. [results] there was a significant difference in the expression of TLR4 mRNA between the control group and the control group. There was no significant difference between the 2 h exposure group and the control group. The expression of RelA mRNA in the control group and the three exposure groups were 0.961.33 / 2.233.04, respectively. The difference among the four groups was statistically significant (蠂 ~ (2 / 2) 6.35) P 0.0048 h exposure group and control group (P < 0.05). There was no significant difference between the 2 h exposure group and the control group. There was a correlation between P0.05 mRNA and RelA mRNA. [conclusion] the expression of TLR4 mRNA and RelA mRNA in lung tissue of mice exposed to silica dust was up-regulated. The results suggest that TLR4/RelA signaling pathway is activated.
【作者单位】：济宁医学院公共卫生学院职业卫生与环境医学重点实验室;济宁市第一人民医院;
【基金】：山东省卫生与计划生育委员会(编号:2014WS0510) 济宁医学院科研计划项目(编号:JY2015KJ003)
【分类号】：R135.2

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