紫菜多糖对脑缺血再灌注损伤保护作用的实验研究

发布时间：2018-03-06 01:31

本文选题：紫菜多糖　切入点：反复脑缺血再灌注　出处：《营养学报》2016年05期 　论文类型：期刊论文

【摘要】：目的研究紫菜多糖(PP)对反复脑缺血/再灌注损伤小鼠的作用。方法采用反复夹闭双侧颈总动脉再灌注法造模,采用避暗法实验测试灌胃给予PP(100、200、400 mg/kg)7 d后对学习记忆障碍的改善作用,同时测定脑组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)及血浆中白细胞介素1β(IL-1β)、IL-6和IL-10的水平,采用透射电镜观察小鼠脑神经元超微结构。结果 PP能明显延长小鼠潜伏期,减少错误次数,提高脑组织SOD含量和GSH-Px活性,降低MDA含量,同时降低血浆IL-1β、IL-6的水平,升高IL-10的水平,连续7 d灌胃给药PP可以显著改善脑缺血再灌注造成的脑神经元损伤。结论 PP对反复脑缺血/再灌注损伤小鼠有保护作用,可能与其抗氧化、降低炎症反应、改善神经元损伤有关。
[Abstract]:Objective to study the effect of porphyra yezoensis polysaccharide (PPN) on repeated cerebral ischemia / reperfusion injury in mice. The improvement of learning and memory impairment was measured by the method of avoiding darkness after intragastric administration of PPX 100g / 200m for 400 mg/kg)7 / d. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) in brain homogenate and the levels of IL-1 尾 and IL-10 in plasma were also measured. Results PP could significantly prolong the latency, reduce the number of errors, increase the content of SOD and GSH-Px, decrease the content of MDA, and decrease the level of plasma IL-1 尾 -IL-6 in mice by transmission electron microscope. Increasing the level of IL-10 and intragastric administration of PP for 7 days could significantly improve the brain neuronal injury induced by cerebral ischemia-reperfusion. Conclusion PP has protective effect on repeated cerebral ischemia / reperfusion injury in mice, which may be associated with antioxidation and decrease of inflammatory reaction in mice with recurrent cerebral ischemia / reperfusion injury. The improvement of neuronal injury is related to.
【作者单位】：河北北方学院药学系;河北北方学院生命科学研究中心;
【基金】：河北省中医药管理局科研计划项目(No.2013078) 河北北方学院自然科学研究计划项目(No.2014013)
【分类号】：R151

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