砷致氧化损伤与p53和p16基因启动子区甲基化的关联研究

发布时间：2018-03-06 23:04

本文选题：砷　切入点：氧化损伤　出处：《环境与健康杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的探讨砷所致的氧化损伤与其所引起的基因启动子区高甲基化的关联。方法于2013年12月,在贵州省燃煤污染型地方性砷中毒病区招募74名常驻居民为研究对象,检测尿砷、发砷及尿中8-羟基脱氧鸟苷(8-OH-d G)的水平以及p53、p16启动子区CpG位点甲基化的情况。结果在控制年龄、性别、吸烟、饮酒后,尿砷与尿中8-OH-d G水平间呈正相关(P0.05)。p16基因启动子区+248位和+317位CpG位点甲基化率与尿砷水平呈正相关(P0.05),+248、+261、+314、+317、+320、+391位CpG位点甲基化率与尿中8-OH-d G水平呈正相关(P0.05)。p53基因启动子区-9位、-127位CpG位点甲基化率与尿中8-羟基脱氧鸟苷水平呈正相关关系(P0.05),+80位CpG位点甲基化率与尿砷水平呈正相关(P0.05)。结论砷介导的氧化应激与砷所致基因异常甲基化间存在一定关联,其相关机制需进一步探索。
[Abstract]:Objective to investigate the oxidative damage induced by arsenic related with promoter hypermethylation. The association method in December 2013, in coal-burning endemic arsenism area in Guizhou province to recruit 74 permanent residents as the research object, the detection of urinary arsenic, arsenic in urine and 8- hydroxydeoxyguanosine (8-OH-d G). The level of p53, p16, CpG at promoter region methylation. Results after controlling for age, sex, smoking, drinking, urine arsenic and urinary 8-OH-d G levels were positively correlated (P0.05).P16 gene promoter +248 and positive +317 CpG sites methylation rate and urinary arsenic levels (P0.05 +248, +261, +314), +317, +320, +391, CpG sites methylation rate was positively correlated with 8-OH-d G levels in urine (P0.05).P53 gene promoter -9, -127, CpG sites methylation rate correlated with urinary 8- hydroxydeoxyguanosine positively relationship (P0.05) CpG, +80 a site There was a positive correlation between basal rate and urinary arsenic level (P0.05). Conclusion arsenic mediated oxidative stress is associated with the abnormal methylation of arsenic, and the related mechanisms need further exploration.

【作者单位】：贵州医科大学环境污染与疾病监控教育部重点实验室;贵州医科大学公共卫生学院;中国人民解放军第44医院;
【基金】：国家自然科学基金(81302394;81430077)
【分类号】：R114

【相似文献】