孕期暴露壬基酚对母鼠雌、孕激素及生产和哺乳的影响

发布时间：2018-03-08 04:18

本文选题：壬基酚　切入点：SD母鼠　出处：《毒理学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的探讨母鼠(SD大鼠)孕期暴露壬基酚(NP)对孕中期及产后雌激素(E_2)、孕激素(P)以及血清NP水平和生产的影响。方法试验分为NP 50、100和200 mg/kg·d和玉米油对照4组。母鼠受孕第6~20 d灌胃染毒NP,分别在孕12 d,分娩后1 d处死母鼠,取血清,化学发光法检测E_2、P水平,液相色谱法检测NP含量,并观察母鼠生产及产后哺乳的相关指标。结果孕12 d,与对照组比较,低、中、高剂量组大鼠血清中NP、E_2、P及E_2/P差异有统计学意义(P0.01)。孕12 d,低、中和高剂量组大鼠血清中NP和E_2高于对照组、P低于对照组(P0.01)。分娩后1 d,与对照组比较,低、中、高剂量组母鼠血清中NP、E_2差异有统计学意义(分娩后1 d,低、中、高剂量组母鼠血清中NP、E_2均高于对照组,P0.01)。各染毒组母鼠未能正常生产和产后不哺乳数量均高于对照组,低剂量组和中剂量组仔鼠窝重均小于对照组(P0.05),与对照组比较,中剂量组和高剂量组的雌雄比例较大(P0.05)。结论孕期暴露NP,可降低孕鼠的体质量,血清NP的含量随暴露剂量增高而升高,干扰孕鼠体内E_2、P水平,导致孕鼠不能正常生产和产后不哺乳,增加F1代仔鼠雌雄比例。
[Abstract]:Objective to investigate the effects of nonylphenol (NPN) exposure during pregnancy on the levels of serum NP and production of estradiol in the second trimester and postpartum of SD rats. Methods the experiment was divided into 4 groups: NP 50,100 and 200 mg/kg 路d and corn oil. NPs were administered intragastrically on the 6th day of gestation and killed on the 12th day of gestation and 1 day after delivery. Serum samples were collected. The levels of E _ 2P were detected by chemiluminescence method. NP content was detected by liquid chromatography, and the relative indexes of maternal production and postpartum lactation were observed. Results at the 12th day of pregnancy, compared with the control group, the serum levels were lower and lower. There was significant difference in serum NPP and ESP between high dose group and high dose group (P 0.01). At the 12th day of pregnancy, the levels of NP and ESP in the middle and high dose groups were higher than those in the control group (P < 0.01). 1 day after delivery, the levels of NP and E _ 2 in the serum of the rats in the control group were lower and lower than those in the control group. There was a statistically significant difference in serum NPD E2 between high dose group and high dose group (1 day after delivery, 1 day after delivery, 1 day after delivery, medium dose group, high dose group all higher than control group (P 0.01). The number of normal production and postpartum unlactation of each exposed group was higher than that of control group. Compared with the control group, the weight of litter in the low dose group and the middle dose group was lower than that in the control group. Compared with the control group, the proportion of female and male in the middle dose group and the high dose group was larger than that in the control group. Conclusion exposure to NPs during pregnancy can reduce the body weight of pregnant mice. The content of serum NP increased with the increase of exposure dose, which interfered with the level of E _ 2P in pregnant rats, which led to the abnormal production of pregnant mice and non-lactation of postpartum, and increased the ratio of male and female of F1 offspring.
【作者单位】：遵义医学院公共卫生学院;遵义医学院附属医院核医学科;遵义医学院附属医院胃肠外科;
【基金】：国家自然科学基金(81360439,81560527) 贵州省科技厅基金(黔科合LH字[2014]7543,[2015]7521号,黔科合J字[2014]2177号,[2014]2185号) 遵义医学院招标课题(2013F-68,2015F-784) 贵州省教育厅青年基金(黔教合KY字[2013]198) 遵义医学院2015重点学科建设经费贵州省卫生厅基金(gzwkj20131127)
【分类号】：R114

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