魔芋低聚糖对结肠炎大鼠肠道菌群的影响

发布时间：2018-03-08 07:36

本文选题：魔芋低聚糖　切入点：溃疡性结肠炎　出处：《中国食品学报》2017年06期 　论文类型：期刊论文

【摘要】：目的:研究魔芋低聚糖(KOS)对溃疡性结肠炎大鼠肠道菌群结构的影响,并探讨其改善大鼠结肠炎症的可能作用机制。方法:采用2,4,6-三硝基苯磺酸(TNBS)诱导大鼠溃疡性结肠炎模型,40只SD大鼠随机被分为4组:正常组、模型组、KOS低剂量组(灌胃1 g/(kg·d))、KOS高剂量组(灌胃4 g/(kg·d))。每日对大鼠进行临床活动度观察,14 d后10%水合氯醛麻醉处死大鼠,无菌收集结肠内容物,制作粪涂片进行菌群失调分度检验,涂布平板法测大肠杆菌、肠球菌、双歧杆菌、乳酸杆菌的数量,气相色谱(GC)检测乙酸、丙酸、丁酸含量。结果:低、高剂量KOS组均明显缓解溃疡性结肠炎大鼠临床症状,改善菌群失调状况,显著提高双歧杆菌、乳杆菌的数量,降低大肠杆菌、肠球菌的数量,并能显著提高短链脂肪酸(SCFA)的产量。结论:魔芋低聚糖可通过调节肠道菌群的结构与功能对溃疡性结肠炎起干预与治疗作用。
[Abstract]:Objective: To study the effect of konjac oligosaccharides (KOS) on ulcerative colitis in rats of intestinal microflora, improve the symptoms of colitis and explore its possible mechanism in rats. Methods: using 2,4,6- three trinitrobenzene sulfonic acid (TNBS) induced rat model of ulcerative colitis, 40 SD rats were randomly divided into 4 groups: the normal group, model group, KOS low dose group (intragastric administration of 1 g/ (kg - D) KOS), high dose group (intragastric administration of 4 g/ (kg - D)). The daily clinical activity of the rat was observed after 14 d of 10% chloral hydrate anesthetized rats were sacrificed and collected in sterile colon content things made of fecal flora indexing smear test, measurement of Escherichia coli, coated plate method Enterococcus, Bifidobacterium, Lactobacillus number, gas chromatography (GC) detection of acetic acid, propionic acid, butyric acid content. Results: low and high dose KOS groups were significantly alleviated in rats with ulcerative colitis clinical symptoms, improve flora, increased significantly The number of bifidobacteria and lactobacilli decreased the number of Escherichia coli, enterococci, and significantly increased the yield of short chain fatty acids (SCFA). Conclusion: konjac oligosaccharides can intervene and treat ulcerative colitis by regulating the structure and function of intestinal flora.

【作者单位】：北京联合大学功能食品科学技术研究院北京联合大学应用文理学院;
【基金】：“十二五”国家科技支撑计划课题(2012BAD33B06)
【分类号】：R151

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