高饱和脂肪饮食对老年大鼠学习记忆和脑Aβ生成的影响及机制

发布时间：2018-03-11 08:52

本文选题：高饱和脂肪　切入点：学习记忆　出处：《中国老年学杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的观察高饱和脂肪饮食对老年大鼠学习记忆和脑β-淀粉样蛋白(Aβ)生成的影响及机制。方法 26只雄性老年大鼠随机分为对照组和高饱和脂肪组各13只,对照组大鼠给予正常饲料,高饱和脂肪组大鼠给予含15%牛油饲料,喂养3个月。用Morris水迷宫测定大鼠学习记忆能力,用酶联免疫吸附法(ELISA)测定海马Aβ含量,用免疫组织化学染色检测脑内β-淀粉样前体蛋白(APP)表达,用黄嘌呤氧化酶法测定血清及脑组织总超氧化物歧化酶(SOD)活性。结果与对照组相比,高饱和脂肪组大鼠平均逃避潜伏期明显延长(P0.05),穿越原平台所在位置次数显著减少(P0.01),脑皮质和海马区APP蛋白表达明显增多(P0.01,P0.05),海马Aβ含量显著升高(P0.01),血清及脑组织总SOD水平均显著降低(P0.01,P0.05)。结论高饱和脂肪饮食降低学习记忆能力、增加APP和Aβ水平,其机制可能与降低脑抗氧化能力有关。
[Abstract]:Objective to observe the effect and mechanism of high saturated fat diet on learning and memory and 尾 -amyloid A 尾 production in aged rats. Methods Twenty-six male aged rats were randomly divided into control group and hypersaturated fat group (n = 13 each). The rats in the control group were given normal diet, the rats in the high saturated fat group were fed with 15% butter diet for 3 months, the learning and memory abilities of the rats were measured by Morris water maze, and the A 尾 content of hippocampus was measured by Elisa. The expression of 尾 -amyloid precursor protein (app) was detected by immunohistochemical staining, and the activity of total superoxide dismutase (SOD) in serum and brain tissue was determined by xanthine oxidase method. In the hypersaturated fat group, the average escape latency was significantly prolonged, the number of times of crossing the site of the original platform was significantly decreased, the expression of APP protein in cortex and hippocampus was significantly increased, the A 尾 content in hippocampus was significantly increased, and the total serum and brain tissue were increased. The levels of SOD were significantly lower than that of P0.01P0.050.Conclusion High saturated fat diet can decrease the ability of learning and memory. The mechanism of increasing the levels of APP and A 尾 may be related to the decrease of antioxidant ability of brain.
【作者单位】：新乡医学院生物化学与分子生物学教研室;新乡医学院护理学院;新乡医学院公共卫生学院;
【基金】：国家自然科学基金资助项目(No.81500984) 河南省重点科技攻关项目(No.152102310112)
【分类号】：R151

【相似文献】