纳米羟基磷灰石对3种正常组织细胞的DNA损伤作用研究

发布时间：2018-03-12 13:10

本文选题：纳米羟基磷灰石　切入点：DNA损伤　出处：《现代预防医学》2017年18期 　论文类型：期刊论文

【摘要】：目的探讨纳米羟基磷灰石(HA-NPs)对3种正常组织细胞的DNA损伤作用,为HA-NPs毒性机制提供实验依据。方法以正常人肝细胞(L02)、肺支气管上皮细胞(HBE)和小鼠成骨细胞(MC3T3)为研究对象,以不同浓度HA-NPs染毒细胞24 h,采用MTT比色法检测细胞毒性,同时用彗星实验和荧光探针法分别检测HA-NPs对细胞的DNA损伤和活性氧水平。结果 HA-NPs在较低浓度(0~400μg/ml)时促进3种细胞的生长,在大于400μg/ml时则抑制细胞生长。随着HA-NPs浓度的增高,3种细胞的拖尾细胞数、尾部DNA百分比、Olive尾距(OTM)均逐渐增加,与对照组比较差异有统计学意义(P0.05)。3种细胞的活性氧荧光强度均随HA-NPs浓度增加而增加,相较于对照组差异有统计学意义(P0.05)。结论 HA-NPs可引起3种受试细胞DNA损伤,其机制可能与HA-NPs诱导细胞产生活性氧有关。
[Abstract]:Objective to investigate the DNA damage effect of HA-NPs on three kinds of normal tissue cells, and to provide experimental evidence for the mechanism of HA-NPs toxicity. Methods normal human hepatocytes (L02), lung bronchial epithelial cells (HA-NPs) and mouse osteoblasts (MC3T3) were used as subjects. The cytotoxicity of cells exposed to different concentrations of HA-NPs for 24 h was detected by MTT colorimetry. At the same time, comet assay and fluorescence probe method were used to detect DNA damage and reactive oxygen species level of HA-NPs. Results HA-NPs promoted the growth of three kinds of cells at a low concentration of 400 渭 g / ml. The cell growth was inhibited when the concentration of HA-NPs was greater than 400 渭 g / ml. With the increase of HA-NPs concentration, the percentage of tail DNA increased gradually. Compared with the control group, the fluorescence intensity of reactive oxygen species increased with the increase of HA-NPs concentration, and the difference was statistically significant compared with the control group. Conclusion HA-NPs can cause DNA damage in three kinds of cells. The mechanism may be related to the production of reactive oxygen species (Ros) induced by HA-NPs.
【作者单位】：四川大学华西公共卫生学院(华西第四医院);四川大学生物材料中心;
【基金】：国家自然科学基金面上项目(NO.81372945)
【分类号】：R114

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