2013-2016年南宁市外环境禽流感病毒监测与分析

发布时间：2018-03-16 00:20

本文选题：外环境　切入点：禽流感病毒　出处：《现代预防医学》2017年20期 　论文类型：期刊论文

【摘要】：目的了解南宁市外环境标本中禽流感病毒的动态分布情况,为人感染禽流感病毒提供风险评估依据。方法采集环境标本用实时荧光PCR法检测A型流感病毒通用型、H5、H7和H9亚型核酸,监测数据用SPSS21.0软件进行统计分析。结果共采集外环境标本468份,A型流感病毒通用型核酸阳性标本共62份,总阳性率为13.25%;H5、H9、A型未分型和H5+H9检出率分别为4.91%、3.42%、4.27%和0.64%。1月、4月和8月禽流感病毒的阳性率较高。各类监测标本中,砧板表面检出率最高(38.10%),粪便最低(7.32%)。结论南宁市城乡活禽市场外环境中有H5、H9禽流感病毒污染,存在感染人的风险,应继续加强外环境禽流感病毒监测。
[Abstract]:Objective to investigate the dynamic distribution of avian influenza virus in environmental samples from Nanning city. Methods Real-time fluorescence PCR assay was used to detect the nucleic acid of H5H7 and H9 subtypes of influenza A virus. The monitoring data were statistically analyzed by SPSS21.0 software. Results A total of 468 samples of influenza A virus positive nucleic acid were collected, 62 of them were positive for common type nucleic acid of influenza A virus. The total positive rate was 13.25% and the positive rate of H5H9 type A and H5H9 were 4.91% and 0.64.1%, respectively. The positive rates of avian influenza virus in April and August were higher. The highest detection rate on the cutting board was 38.10 and the lowest in feces was 7.320.Conclusion there is H5H9 avian influenza virus contamination in the environment of live poultry market in urban and rural areas of Nanning, and there is a risk of human infection, so we should continue to strengthen the surveillance of avian influenza virus in external environment.
【作者单位】：南宁市疾病预防控制中心;
【分类号】：R181.8;R511.7

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