青春期低剂量氰戊菊酯暴露对小鼠行为的影响

发布时间：2018-03-16 09:27

本文选题：青春期　切入点：低剂量　出处：《安徽医科大学学报》2015年02期 　论文类型：期刊论文

【摘要】：目的探究青春期低剂量氰戊菊酯暴露对小鼠行为的影响。方法对出生后第28天的48只ICR小鼠雌雄各半分别进行不同浓度氰戊菊酯(0、0.02、0.20、2.00 mg/kg)灌胃染毒。出生后第28~56天。在出生后第9周,雌雄小鼠分别进行相关行为学实验。通过黑白巷实验、旷场实验检测小鼠的探索行为和焦虑相关行为,采用Morris水迷宫实验检测小鼠的空间学习和记忆相关行为。结果各组小鼠在旷场实验中的行为差异无统计学意义。在黑白巷实验中,与0 mg/kg组雄性小鼠比较,0.02 mg/kg组和2.00 mg/kg组雄性小鼠跨过中线的潜伏期明显延长(P=0.006,P=0.039)。在Morris水迷宫的定位航行实验中,与0 mg/kg组雌鼠比较,0.02 mg/kg组和2.00 mg/kg组的雌鼠登陆平台的潜伏期明显延长(P=0.011,P=0.014);与0 mg/kg组雌鼠比较,0.20 mg/kg组的雌鼠搜寻平台的距离明显增加(P=0.003);而雄性小鼠各组指标之间差异无统计学意义。在Morris水迷宫的空间探索实验中,与0 mg/kg组比较,0.20mg/kg组雌鼠的目标象限时间比降低(P=0.046);而雄鼠各组指标之间的差异无统计学意义。结论青春期低剂量的氰戊菊酯暴露能够影响小鼠的多种行为,可能影响小鼠青春期的神经行为发育。
[Abstract]:Objective to investigate the effects of low dose fenvalerate exposure on behavior in mice. Methods 48 ICR mice were treated with different concentrations of fenvalerate 0.20 0. 20 mg / kg by gavage on the 28th day after birth. In the ninth week after birth, The related behavioral experiments were carried out in male and female mice. The exploratory behavior and anxiety related behavior of mice were detected by black and white lane experiment and open field experiment. Morris water maze test was used to detect spatial learning and memory-related behaviors in mice. Results there was no significant difference in the behavior of each group in the open field experiment. Compared with the male mice of 0 mg/kg group, the incubation period of 0. 02 mg/kg group and 2. 00 mg/kg group male mice crossed the midline was significantly prolonged. In the navigation experiment of Morris water maze, the incubation period of 0. 02 mg/kg group and 2. 00 mg/kg group was significantly prolonged. Compared with 0 mg/kg group, the latency of female rat landing platform in 0 mg/kg group and 2 00 mg/kg group was significantly prolonged, and the distance of search platform in 0. 20 mg/kg group was significantly longer than that in 0 mg/kg group, while the difference between male group and female group was significant. No statistical significance. In the space exploration experiment of Morris water maze, Compared with 0 mg / kg group, the target quadrant time ratio of female rats in 0.20 mg / kg group was decreased by 0.046%, but there was no significant difference between male and female groups. Conclusion low dose fenvalerate exposure in puberty can affect the behavior of mice. It may affect the neurobehavioral development of puberty in mice.
【作者单位】：安徽医科大学公共卫生学院儿少与卫生保健学系;安徽医科大学公共卫生学院卫生毒理学系;
【基金】：安徽省自然科学基金(编号:KJ2010A173) 国家自然科学基金(编号:81102155) 安徽医科大学博士科研课题资助(编号:XJ201115)
【分类号】：R114

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