纸片法在托幼机构环境菌落总数检测中的应用研究

发布时间：2018-03-16 11:03

本文选题：纸片法　切入点：托幼机构　出处：《中国消毒学杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的比较纸片法与传统平皿法对托幼机构环境菌落总数检测的效果差异,分析两者相关性,以寻找一种简便、快捷、准确的检测方法。方法分别采用纸片法和平皿法对托幼机构工作人员手、物体表面、室内空气等样本进行检测,用纸片直接接触法和棉签涂抹法对相同物体表面采样,同时用纸片法暴露不同的时间检测室内空气沉降菌,用配对t检验分析不同检测和采样方法及暴露时间之间的结果差异。结果纸片法与平皿法在检测工作人员手和物体表面样品菌落总数时基本一致,差异无统计学意义(P0.05),用纸片直接接触法对物体表面采样的检测结果明显高于棉签涂抹法,两者之间差异有显著性(P0.05)。纸片法与平皿法在检测空气菌落总数方面48 h结果无明显差异,但24 h纸片菌落计数结果明显高于平皿。纸片暴露法采样3、5、10和15 min的计数结果逐渐降低,10 min时的检测结果最接近平皿法。结论纸片法在检测环境菌落总数方面与平皿法相关性、准确性较高,且简便、快捷,避免了大量前期准备和后期清洁消毒处理工作,有一定应用和推广价值。
[Abstract]:Objective to compare the effect of paper plate method and traditional plate method on the detection of total number of bacteria in nursery environment, and to analyze the correlation between the two methods in order to find a simple and fast method. Methods the hands, surfaces, indoor air and other samples of kindergarten workers were detected by using the paper plate method and the cotton swabs smear method, respectively, and the same objects were sampled by the paper direct contact method and the cotton swabs smear method. At the same time, the paper method was used to detect indoor air sedimentation bacteria at different time. The results of different detection and sampling methods and exposure time were analyzed by paired t test. Results the results showed that the paper method and the plate method were basically consistent in the detection of the total number of bacteria on the workers' hands and on the surface of objects. The difference was not statistically significant (P 0.05). The results of surface sampling by direct contact method were significantly higher than that by cotton swabs smear method. There was a significant difference between the two methods (P 0.05). There was no significant difference between the paper plate method and the plate method in the total number of bacteria in the air for 48 h. However, the results of 24 h disk colony count were significantly higher than that of flat dish. The results of disk exposure sampling for 3 ~ 5 ~ 10 and 15 min decreased gradually, and the results at 10 min ~ 10 min were close to those of plate method. Conclusion the paper method is closely related to the plate method in detecting the total number of bacteria in the environment. The accuracy is high, simple and fast, which avoids a large amount of pre-preparation and post-cleaning and disinfection work, and has certain application and promotion value.
【作者单位】：天津市北辰区疾病预防控制中心;
【基金】：天津市北辰区科技发展计划项目(2015-SHGY-03)
【分类号】：R175

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