荧光分子印记纳米传感器对乳制品中痕量三聚氰胺快速检测

发布时间：2018-03-17 23:24

本文选题：分子印记技术　切入点：纳米硅胶　出处：《南京医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：本文设计将荧光功能单体2-丙烯酰胺基喹啉植入印记聚合物中,对结合位点进行荧光标记,制备兼有分子识别和荧光信号输出的传感器。具体合成步骤为：首先,以正硅酸四乙酯为原料合成粒径为250 nm左右的纳米硅胶小球。其次,应用烷基化试剂3-(甲基丙烯酰氧)丙基三甲氧基硅烷在小球表面修饰。最后,将修饰后的硅胶小球与模板分子(三聚氰胺)、复合功能单体(2-丙烯酰胺基喹啉和甲基丙烯酸)混合,在交联剂(乙二醇二甲基丙烯酸酯)作用下聚合,制备出对三聚氰胺具有高选择性和高分离效率的荧光分子印记纳米硅胶。透射电镜和吸附性能实验表明,该粒子表面具有可控的印记壳厚度(～6 nm),且对三聚氰胺有较高的亲和力(最大吸附量为70.20μmol g-1),最大吸附量大约是非印记粒子的4.5倍。当该粒子被用作固相萃取材料和荧光传感器时,通过荧光增强原理,初步实现对乳制品(牛奶、酸奶和冰淇林)中痕量三聚氰胺的高选择性和高灵敏性检测,它们的加样回收率和相对标准偏差分别为94.8%(0.7%),98.5%(2.5%)和102.1%(2.2%)。因此,该方法可为食品中非法添加剂(非荧光性物质)检测提供参考。
[Abstract]:In this paper, the fluorescent functional monomer 2-acrylamide quinoline was implanted into imprinted polymer, the binding site was labeled with fluorescence, and a sensor with both molecular recognition and fluorescence signal output was prepared. Nano-silica colloidal spheres of about 250nm were synthesized from tetraethyl orthosilicate. Secondly, alkylation reagent 3-( methacryloxy) propyltrimethoxy silane was used to modify the surface of the spheres. The modified silica gel spheres were mixed with template molecules (melamine, complex functional monomers, 2-acrylamide quinoline and methacrylic acid) and polymerized under the action of crosslinking agent (ethylene glycol dimethacrylate). Fluorescent molecular imprinted nano-silica gel with high selectivity and high separation efficiency for melamine was prepared. The surface of the particle has a controllable shell thickness of 6 nm and a high affinity to melamine (the maximum adsorption capacity is 70.20 渭 mol g -1), and the maximum adsorption capacity is about 4.5 times that of the non-imprinted particle. When the particle is used as a solid phase extraction material and a fluorescence sensor, The high selectivity and high sensitivity of trace melamine in dairy products (milk, yoghurt and ice cream) were preliminarily realized by fluorescence enhancement principle. The recoveries and relative standard deviations of trace melamine in dairy products (milk, yoghurt and ice cream) were 94.80.77 and 98.52.5, respectively. The method can provide a reference for the detection of illegal additives (non-fluorescent substances) in food.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R155.5

【相似文献】