虾青素油剂干预NMBzA诱导的F344大鼠食管癌效果及机制研究

发布时间：2018-03-19 03:11

本文选题：虾青素　切入点：食管癌　出处：《郑州大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的探讨虾青素油剂抑制食管癌的效果,并初步阐明其抑制食管癌发生的机制。方法取84只4-6周龄F344雄性大鼠,随机分为6组,即对照组A[0.25mg/kg·body weight(BW)],[双蒸水+20%二甲基亚砜(Dimethyl sulfoxid,DMSO)],虾青素低剂量组B(10mg/kg·BW)、中剂量组C(50mg/kg·BW)、高剂量组D(100mg/kg·BW)、溶剂组E(红花籽油)、暴露组F[N-甲基苄基亚硝胺,(N-nitrosomethylbenzylamine,NMBzA)+DMSO]。B、C、D、E、F组皮下注射NMBzA(0.25mg/kg·BW)(5周),B、C、D组灌胃虾青素油剂(25周),每周三次。实验第25周、35周分别处死大鼠,腹主动脉采血,HE染色观察食管组织形态结构,酶联免疫法检测血清超氧化物歧化酶(Superoxide dismutase,T-SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidases,GSH-PX)活性、丙二醛(Malondialdehyde,MDA)含量及血浆过氧化氢酶(Catalase,CAT)活性、总抗氧化能力(Total antioxidant capacity,TAOC)及血清转化生长因子β1(Transforming Growth Factor-beta 1,TGF-β1)水平。用SPSS 21.0统计软件对实验数据进行分析,检验水准α=0.05。结果(1)大鼠食管肉眼观肿瘤发生情况:第25周,溶剂组、暴露组发生率均高于对照组及中剂量组,暴露组发生率高于低剂量组。第35周,溶剂组和暴露组发生率均高于对照组。差异均具有统计学意义(P0.05)(2)大鼠食管黏膜上皮组织学发现:第25周和35周,低剂量组、高剂量组、溶剂组及暴露组组织学形态与对照组不同,低剂量组及高剂量组多见异常增生、溶剂组及暴露组多见肿瘤,对照组仅见正常上皮和单纯增生。第25周,中剂量组仅见正常上皮和单纯增生,与低剂量组、高剂量组、溶剂组及暴露组组织学形态不同,但第35周中剂量组可见异常增生和肿瘤,与暴露组组织学形态不同。差异均具有统计学意义(P0.05)。(3)大鼠氧化应激水平检测:第25周,对照组、中剂量组血清GSH-PX活性均高于暴露组。高剂量组和对照组血浆CAT活性均高于暴露组。暴露组血清MDA含量高于低剂量组。中剂量组血浆TAOC水平均高于暴露组及溶剂组。差异均有统计学意义(P0.05)。(4)大鼠血清TGF-β1水平检测:第25周,中剂量组及暴露组均高于低剂量组及对照组。第35周,溶剂组及暴露组水平高于对照组,差异均有统计学意义(P0.05)。(5)大鼠体重及脾脏重量:第25周,与对照组、低剂量组、中剂量组相比,高剂量及暴露组体重较轻;中剂量组大鼠脾脏重量高于溶剂组及暴露组,差异均有统计学意义(P0.05)。结论适当剂量虾青素油剂能够抑制NMBzA诱导的大鼠食管癌发生,可能通过提高大鼠抗氧化能力,提高大鼠TGF-β1的水平而抑制食管癌的发生。
[Abstract]:Objective to investigate the inhibitory effect of astaxanthin oil on esophageal carcinoma and its mechanism. Methods 84 F344 male rats aged 4-6 weeks were randomly divided into 6 groups. Control group A [0.25 mg / kg 路body weighted BW], [double distilled water 20% Dimethyl sulfoxidido], astaxanthin low dose group B10 mg / kg 路BWN, middle dose group C0 50 mg / kg 路BWW, high dose group D 100 mg / kg 路BW, solvent group E, exposure group F [N-nitrothylbenzylamine NMBzA]. The rats were killed at the 25th week, 35 weeks after the experiment. The morphological structure of esophagus was observed by HE staining of abdominal aorta. The activities of superoxide dismutase T-SODX, glutathione peroxidase peroxidase (Glutathione peroxidase) GSH-PX, malondialdehyde (malondialdehyde) malondialdehyde (MDA) and plasma catalase catalase (CAT) in serum were detected by enzyme-linked immunosorbent assay (Elisa). Total antioxidant capacity (TAOC) and serum transforming Growth Factor-beta (TGF- 尾 1) levels of TGF- 尾 1. The experimental data were analyzed with SPSS 21.0 software to test the level of 伪 0. 05. The results showed that the incidence of naked esophageal neoplasms in rats: 25 weeks, solvent group, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1, TGF- 尾 1 and TGF- 尾 1. The incidence of exposure group was higher than that of control group and middle dose group, and the incidence of exposure group was higher than that of low dose group. The incidence of esophageal mucosal epithelium in solvent group and exposure group was higher than that in control group. The difference was statistically significant (P 0.05): at the 25th week and the 35th week, the low dose group and the high dose group, the lower dose group, the higher dose group, the lower dose group, the lower dose group and the higher dose group. The histological morphology of solvent group and exposure group was different from that of control group. Abnormal hyperplasia was found in low dose group and high dose group, tumor in solvent group and exposure group, normal epithelium and simple hyperplasia in control group. Normal epithelium and simple hyperplasia were observed in the middle dose group, which were different from those in the low dose group, the high dose group, the solvent group and the exposure group, but abnormal hyperplasia and tumor were found in the middle dose group at the 35th week. The difference was statistically significant (P 0.05)) the level of oxidative stress was detected in rats: at the 25th week, the control group, the control group, and the control group. The serum GSH-PX activity in the middle dose group was higher than that in the exposure group, the plasma CAT activity in the high dose group and the control group was higher than that in the exposure group, the serum MDA level in the exposure group was higher than that in the low dose group, and the plasma TAOC level in the middle dose group was higher than that in the exposure group and solvent group. The levels of TGF- 尾 1 in serum of rats were detected at the 25th week. At the 35th week, the level of the solvent group and the exposure group were higher than that of the control group, and the difference was statistically significant (P < 0.05) the weight and spleen weight of the rats: at the 25th week, it was higher than that in the control group and the low-dose group. The weight of spleen in the middle dose group was higher than that in the solvent group and exposure group, and the difference was statistically significant (P 0.05). Conclusion the appropriate dosage of astaxanthin oil can inhibit the occurrence of esophageal carcinoma induced by NMBzA in rats. It is possible to inhibit the occurrence of esophageal cancer by increasing the level of TGF- 尾 1 and anti-oxidation ability in rats.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R151

【参考文献】