基于SERS技术检测牛奶中氨苄西林的研究

发布时间：2018-03-19 14:06

本文选题：表面增强拉曼光谱　切入点：氨苄西林　出处：《中国抗生素杂志》2017年09期 　论文类型：期刊论文

【摘要】：目的利用表面增强拉曼光谱(SERS)检测牛奶样品中的氨苄西林,对其进行定性及半定量分析。方法首先构建了新型氧化铝陶瓷SERS检测平台。其次对氨苄西林的拉曼特征峰进行了归属,并确定了最佳的实验条件。然后对水溶液中的氨苄西林进行了SERS检测,并获得了检测限。最后进行了牛奶样品中的氨苄西林的检测,并利用线性拟合实现了对加标牛奶样品中氨苄西林的半定量分析。结果牛奶样品中的氨苄西林的检测限为10-3g/L,在10-3~10-1g/L浓度范围内,拉曼特征峰1000cm-1的强度随牛奶样品中氨苄西林的浓度的线性关系为y=23408.93x+1801.47,相关系数为0.993,回收率在95.0%~110.0%之间。结论包括对牛奶样品的预处理过程(约5min)在内,整个SERS检测过程不超过7min。该方法对牛奶中的污染物进行定量和定性分析具有广阔的应用前景。
[Abstract]:Objective to detect ampicillin in milk samples by surface enhanced Raman spectroscopy (SERS). Qualitative and semi-quantitative analysis were carried out. Firstly, a new alumina ceramic SERS detection platform was constructed. Secondly, the Raman characteristic peaks of ampicillin were assigned. The optimum experimental conditions were determined. Then ampicillin in aqueous solution was detected by SERS, and the detection limit was obtained. Finally, ampicillin in milk samples was detected. The semi-quantitative analysis of ampicillin in standard milk samples was realized by linear fitting. Results the detection limit of ampicillin in milk samples was 10 ~ (-3) g / L, in the range of 10 ~ (-3) ~ (-1) g 路L ~ (-1) of ampicillin. The linear relationship between Raman characteristic peak 1000cm ~ (-1) and ampicillin concentration in milk samples was yz23408.93x 1801.47, the correlation coefficient was 0.993, and the recovery rate was 95.0 ~ 110.0%. Conclusion the pretreatment process of milk samples (about 5 min) is included in the Raman characteristic peak of 1000 cm ~ (-1), the correlation coefficient is 0.993, and the recovery rate is between 95.0% and 110.0%. The whole process of SERS detection is less than 7 mins. This method has a broad application prospect for quantitative and qualitative analysis of pollutants in milk.
【作者单位】：内蒙古民族大学附属医院;内蒙古民族大学物理与电子信息学院;哈尔滨工业大学可调谐(气体)激光技术国家级重点实验室;
【基金】：国家国际技术合作专项项目(No.2011DFA31770)资助的课题内蒙古民族大学校级科研项目(No.NMDYB1444)
【分类号】：R155.57

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