铅对大鼠骨组织代谢影响

发布时间：2018-03-20 13:29

本文选题：铅中毒　切入点：Pb　出处：《中国公共卫生》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨铅中毒对大鼠机体骨代谢影响及机制。方法选取21日龄SPF级雄性SD大鼠40只,适应性喂养7 d后,随机分为4组:对照组(去离子水)、低、中、高剂量铅组(0.1%、0.2%、0.3%醋酸铅水);饲养60 d后,称重;取大鼠后肢股骨,ICP法测定股骨中Pb、Ca含量;采用常规苏木素-伊红染色法(HE)对大鼠骨组织形态进行观察;应用荧光定量RT-PCR法测定大鼠骨骼中过氧化物酶体增长因子活化受体γ(PPAR-γ)、Wnt2、转录因子1(Tcf-1)、和β-catenin基因表达量。结果与对照组比较,随着染铅剂量增加,低、中、高剂量铅组大鼠骨骼铅含量[分别为(0.221 5±0.033 0)、(0.335 3±0.040 7)、(0.399 2±0.030 9)mg/g]均升高,而骨钙含量[分别为(21.7194±1.120 8)、(21.559 5±1.094 0)、(21.421 7±0.700 2)mg/g]均下降,差异均有统计学意义(P0.05);与对照组比较,低、中剂量铅组大鼠骨小梁相对变薄、变细,高剂量铅组大鼠股骨骨小梁稀疏、变细、变薄或断裂,骨髓腔相对扩大;与对照组比较,高剂量铅组大鼠骨骼中PPAR-γ、Wnt2、Tcf-1及β-catenin m RNA表达量[分别为(0.821 3±0.0202)、(0.852 6±0.030 8)、(0.874 3±0.077 2)、(0.874 5±0.055 3)]均下降,差异均有统计学意义(P0.05)。结论铅对大鼠骨组织中钙的吸收具有抑制作用,对骨组织结构具有损伤作用,其机制可能与下调骨骼中PPAR-γ、Wnt2、Tcf-1及β-catenin基因表达有关。
[Abstract]:Objective to investigate the effect and mechanism of lead poisoning on bone metabolism in rats. Methods Forty SPF grade male SD rats aged 21 days were selected and fed adaptively for 7 days, then were randomly divided into 4 groups: control group (deionized water, low, middle and low). In the high dose lead group, 0.3wt% lead acetate water was used to measure the content of Pb-Ca in the femur of the hind limbs of rats, and the bone morphology of rats was observed by routine hematoxylin-eosin staining method. The expression of peroxisome growth factor-activated receptor (PPAR- 纬) Wnt2, transcriptional factor 1tcf-1, and 尾 -catenin in rat bone was determined by fluorescence quantitative RT-PCR. Results compared with the control group, the expression of peroxisome growth factor-activated receptor 纬 -PPAR- 纬 nt2, transcription factor 1- Tcf-1, and 尾 -catenin were measured. The bone lead content in the high dose lead group [0.221 5 卤0.033 0 + 0.335 3 卤0.040 7 mg / g] increased, while the bone calcium content [21.559 5 卤1.094 021. 421 卤0.700 2 mg / g] decreased significantly, compared with the control group, the difference was significant (P 0.05), and the difference was significant (P < 0.05), which was significantly lower than that in the control group (P < 0.05), and was significantly lower than that in the control group (P < 0.05). The bone calcium content of the rats in the high dose lead group [21.559 5 卤1.094 0 21. 421 卤0 700 2 mg / g, respectively] was significantly lower than that in the control group (P < 0. 05). The trabeculae of the femur were thinned, thinned, thinned, thinned or broken in the middle dose lead group, and the medullary cavity was relatively enlarged in the high dose lead group, and compared with the control group, the bone trabeculae of the middle dose lead group were thinner and thinner than those of the control group. The expression of PPAR- 纬 Wnt2Tcf-1 and 尾 -catenin m RNA in the bone of rats in high dose lead group [0.821.3 卤0.02022 卤0.852 6 卤0.030 8 + 0.874 3 卤0.077 2 + 0. 874 5 卤0.055 3] were all decreased, and the difference was statistically significant (P 0.05). Conclusion lead can inhibit the absorption of calcium in bone tissue of rats. The mechanism may be related to the down-regulation of the expression of PPAR- 纬 Wnt2Tcf-1 and 尾 -catenin in bone.
【作者单位】：山西农业大学食品科学与工程学院;
【基金】：生态畜牧与环境兽医学山西省重点实验室开放基金(KF2013-01) 山西农业大学引进人才博士科研启动费(2013YJ30);山西农业大学科技创新基金(20132-02) 山西省自然科学基金(2010011043-1)
【分类号】：R114

【相似文献】