不同周龄SD大鼠肝脏非肿瘤病变分析

发布时间：2018-03-27 09:49

本文选题：SD大鼠　切入点：肝脏　出处：《中国职业医学》2017年02期

【摘要】：目的探讨不同周龄SD大鼠肝脏非肿瘤病变情况。方法收集亚急性毒性实验、亚慢性毒性实验和慢性毒性与致癌合并实验安全性评价毒理学实验中空白对照组的无特定病原体级SD大鼠,于实验终期即大鼠周龄分别为第10、19、56和108周龄(分别设为10、19、56和108周龄组,每组雌、雄各100只)时处死大鼠,计算肝脏脏器系数,并进行肝脏组织病理诊断,分析不同周龄大鼠肝脏非肿瘤病变情况。结果大鼠肝脏脏器系数在19、56和108周龄组均低于10周龄组(P0.05),在56和108周龄组均低于19周龄组(P0.05),在108周龄组高于56周龄组(P0.05)。10、19、56和108周龄组大鼠肝脏非肿瘤病变在雌性大鼠分别检出6、6、13和15种,在雄性大鼠分别检出6、8、12和17种。无论是雌性还是雄性大鼠,各组大鼠肝细胞脂肪变性、肝细胞水肿和肝脏炎症细胞浸润的发生率均随着周龄的增加而增加(P0.05);56、108周龄组大鼠肝内胆管增生和肝内胆管纤维化的发生率均高于10、19周龄组(P0.008);108周龄组大鼠肝窦扩张的发生率高于19周龄组(P0.008)。结论 SD大鼠肝脏自发性非肿瘤病变较多见,以肝细胞脂肪变性、肝细胞水肿和肝脏炎症细胞浸润为主,且其发生率呈随大鼠周龄的增加而增加的趋势。
[Abstract]:Objective to investigate the nonneoplastic lesions of the liver in SD rats at different weeks of age. Methods Subacute toxicity tests were performed in SD rats. Safety evaluation of subchronic toxicity test and combination of chronic toxicity and carcinogenesis the SD rats with no specific pathogens in the blank control group in toxicology experiment. At the end of the experiment, the rats were killed at the age of 10 ~ 19 ~ (56) and 108 ~ (th) weeks (100 female and 100 male, respectively), and the liver organ coefficient was calculated, and the pathological diagnosis of liver tissue was made. Results the liver organ coefficient of rats at the age of 1956 and 108 weeks was lower than that of the group of 10 weeks of age, and that of the group of 56 and 108 weeks of age was lower than that of the group of 19 weeks of age, and that of the group of 108 weeks of age was higher than that of the group of 56 weeks of age (P0.05.101956). 13 and 15 kinds of liver nonneoplastic lesions were detected in female rats at the age of 108 weeks. In male rats, 12 and 17 species of hepatocytes were detected respectively. Fatty degeneration of hepatocytes was detected in both male and female rats. The incidence of hepatocyte edema and hepatic inflammatory cell infiltration increased with the increase of age. The incidence of intrahepatic bile duct hyperplasia and intrahepatic bile duct fibrosis in the group of 56 108 weeks old was higher than that in the group of 10 ~ 19 weeks of age (P 0.008) and the rate of sinusoidal dilatation in the group of 108 weeks of age was higher than that in the group of 10 ~ 19 weeks of age. Conclusion the incidence of spontaneous nonneoplastic lesions in the liver of SD rats is higher than that in the 19-week-old group. Fatty degeneration of hepatocytes, edema of hepatocytes and infiltration of inflammatory cells in liver were the main factors, and the incidence increased with the increase of weeks of age in rats.
【作者单位】：广东省职业病防治院广东省职业病防治院重点实验室;
【基金】：广东省医学科学技术研究基金(A2016122) 广东省职业病防治重点实验室(2012A061400007)
【分类号】：R135

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