气管滴注纳米二氧化硅在大鼠体内时间分布特征

发布时间：2018-03-29 22:10

本文选题：纳米颗粒　切入点：二氧化硅　出处：《中国职业医学》2017年05期

【摘要】：目的观察纳米二氧化硅(SiO_2)经气管一次性非暴露式滴注后在大鼠体内的时间分布特征。方法无特定病原体级雄性Wistar大鼠随机分为对照组和1、3、5、7、14、21、28 d实验组,每组6只,采用一次性非暴露式气管滴注法,各实验组大鼠均予质量浓度为50.00 g/L的纳米SiO_2混悬液1.0 mL处理,对照组大鼠不予任何处理。分别于染尘后相应时间点采集各组大鼠的血清和肺脏、脾脏、肝脏、肾脏组织,采用电感耦合-等离子体发射光谱法测定样品中硅元素的水平。取大鼠肺组织进行病理学观察,采用偏振光显微镜观察纳米SiO_2颗粒分布情况。结果大鼠肺组织在纳米SiO_2染尘后1~7 d出现以渗出性炎症反应为主的改变,在染尘后14~28 d以增生性炎性病变为主;28 d实验组大鼠肺组织可见细胞性结节。偏振光显微镜下,各实验组大鼠肺脏组织中于染尘后1 d可观察到明显纳米SiO_2颗粒物分布,其后呈现随着观察时间的增加而减少的趋势,至染尘后21和28 d极少见纳米SiO_2颗粒物分布。实验组大鼠血清、肺脏和脾脏组织中硅元素水平均于染尘后1 d升高达到峰值,在染尘后3~7 d(血清)或3~14 d(肺脏、脾脏)呈现时间依赖性的下降,且恢复正常水平。实验组大鼠肝脏和肾脏的硅元素水平在染尘后1~5 d均无出现有统计学意义的升高(P0.05);均于染尘后7 d才出现有统计学意义的升高(P0.05),分别于染尘后14和21 d升高达到峰值,其后下降,至染尘后28 d仍未恢复正常水平。各实验组大鼠的肺脏、脾脏、肝脏和肾脏硅元素水平均高于血清(P0.05);在染尘后1~5 d肺脏、脾脏硅元素水平均高于肝脏和肾脏(P0.05),在染尘后14~28 d肺脏、脾脏硅元素水平均低于肝脏和肾脏(P0.05)。结论纳米SiO_2经呼吸道染尘可导致大鼠出现肺损伤;染尘后1~5 d主要分布于肺脏和脾脏,在14~28 d主要分布于肝脏和肾脏。
[Abstract]:Objective to observe the distribution of time in rats after one dose of non-exposed infusion of nano-SiOSZ. Methods male Wistar rats with no specific pathogens were randomly divided into two groups: control group and experimental group for 28 days, with 6 rats in each group, 6 rats in each group were randomly divided into two groups: control group (n = 6), control group (n = 6) and control group (n = 28). The rats in each experimental group were treated with 1.0 mL of nanometer SiO_2 suspension of 50.00 g / L mass concentration by a single unexposed tracheal drip. Serum and lung, spleen, liver and kidney tissues were collected at the corresponding time points after dusting in the control group. The level of silicon in the samples was determined by inductively coupled plasma emission spectrometry (ICP-AES). The distribution of nanometer SiO_2 particles was observed by polarized light microscope. Results the pulmonary tissues of rats showed mainly exudative inflammatory reaction 1 ~ 7 days after exposure to nanometer SiO_2 dust. Cellular nodules were observed in the lung tissue of rats in the experimental group on the 28th day after exposure to dust. Under polarized light microscope, the distribution of SiO_2 particles was observed in the lung tissue of each experimental group on the 1st day after exposure to dust. The distribution of nanometer SiO_2 particles was rare at 21 and 28 days after dust exposure. The levels of silicon in serum, lung and spleen of the experimental group were all increased to a peak at 1 day after exposure to dust. At 3d (serum) or 3d (lung, spleen) after exposure to dust, there was a time-dependent decrease. The level of silicon in liver and kidney of the experimental group did not increase significantly at 1: 5 days after exposure to dust, and it was only 7 days after exposure to dust that there was a statistically significant increase in the level of silicon in the liver and kidney of the experimental group, respectively, after exposure to dust, there was a significant increase in the level of silicon in the liver and kidney of the experimental group at 7 days after exposure to dust, respectively. On the 14th and 21st days, the increase reached its peak. The levels of silicon in lung, spleen, liver and kidney of rats in each experimental group were all higher than those in serum P0.05, and the levels of silicon in lung of rats in each experimental group were higher than those in serum P0.05, and in the lung of rats at 1 ~ 5 days after exposure to dust, there was no return to normal level at 28 days after exposure to dust. The level of silicon in spleen was higher than that in liver and kidney (P 0.05), and the level of silicon in spleen was lower than that in liver and kidney at 14 ~ 28 days after dusting. Conclusion Nano-scale SiO_2 can cause lung injury in rats. It was mainly distributed in lung and spleen at 1 day after dusting, and in liver and kidney on day 1428.
【作者单位】：华北理工大学基础医学院;华北理工大学医学实验研究中心;国家科技部老年医学国际科技合作基地;
【基金】：河北省科学技术研究与发展计划项目(10276116D) 华北理工大学大学生创新项目(X2015143)
【分类号】：R114

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