成瘾剂量下烟碱对大鼠的毒性损伤

发布时间：2018-03-31 14:21

本文选题：烟碱　切入点：皮下注射　出处：《烟草科技》2017年07期

【摘要】：为研究成瘾剂量下烟碱对大鼠的毒性作用,采用皮下注射方式和条件位置偏好(CPP)装置构建了SD大鼠的烟碱依赖模型,考察了成瘾初期和持续期SD大鼠对烟碱依赖的变化,并对烟碱成瘾大鼠血常规、血生化等生理指标以及各器官的病理学改变进行了研究。结果表明:(1)采用0.2 mg/kg给药剂量,在第5次给药周期后,大鼠形成了稳定的烟碱依赖,在持续给药的过程中大鼠对烟碱的依赖程度呈现先升高后与成瘾初期持平的趋势。(2)烟碱能引起大鼠炎症相关指标嗜酸性粒细胞百分率(EOS)表达量下调,以及肝脏损伤相关指标γ-谷氨酰转肽酶(GGT)及总胆红素(TBIL)的上调;(3)烟碱暴露在一定程度上能引起大鼠的肾脏和肝脏轻度水肿;肺部有较明显病理症状,且以炎症为主。因此,成瘾剂量(0.2 mg/kg)暴露下烟碱会对大鼠产生轻度的毒性,对肺部、肝脏及免疫系统造成一定的毒性损伤。
[Abstract]:In order to study the toxic effect of nicotine on rats at addictive dose, the nicotine dependence model of SD rats was established by subcutaneous injection and conditional position preference (CPP) device. The changes of nicotine dependence in SD rats during the initial and continuous periods of addiction were investigated.The physiological indexes such as blood routine, blood biochemistry and pathological changes of each organ were studied in rats with nicotine addiction.The results showed that after 0. 2 mg/kg administration, the rats developed a stable nicotine dependence after the fifth administration cycle.During the course of continuous administration, the dependence on nicotine in rats increased first and then remained the same as in the initial stage of addiction. 2) nicotine could induce the down-regulation of eosinophils percentage of eosinophils (EOS) expression, which was related to inflammation in rats.The up-regulation of 纬 -glutamyl transpeptidase (GGTT) and total bilirubin TBIL (TBIL) in liver injury induced mild edema in kidney and liver in rats to some extent.Therefore, exposure to nicotine at an addictive dose of 0.2 mg / kg can cause mild toxicity to rats, as well as toxic damage to lungs, liver and immune system.
【作者单位】：中国科学院合肥物质科学研究院应用技术研究所;中国科学技术大学科学岛分院;国家烟草质量监督检验中心;郑州大学基础医学院;
【基金】：国家烟草专卖局重点项目“烟碱致瘾性阈值研究”(110201402037) 国家烟草质量监督检验中心科技创新项目“电子烟主成分气溶胶吸入暴露毒性研究”(552015CQ0060)
【分类号】：R114;TS41

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