纳米硫化铅对大鼠学习记忆能力及海马组织中铜锌含量的影响

发布时间：2018-04-01 04:13

本文选题：纳米硫化铅　切入点：学习记忆　出处：《环境与健康杂志》2015年07期

【摘要】：目的探讨纳米硫化铅染毒对大鼠学习记忆能力及海马组织中铜、锌含量的影响。方法将24只健康成年SPF级SD雄性大鼠随机分为3组,分别为对照(高温灭菌生理盐水)组和15、30 mg/kg纳米硫化铅染毒组,每组8只。采用气管注入法进行染毒,染毒容积为5 ml/kg,每周1次,连续染毒3个月。采用Morris水迷宫实验检测大鼠的学习记忆能力,测定全血和海马组织中铅含量以及海马组织中铜、锌含量及其比值。结果与对照组比较,各剂量纳米硫化铅染毒大鼠的逃避潜伏期和首次到达平台时间均延长,停留目的象限时间缩短,穿越平台次数减少,全血和海马组织中的铅含量均较高,海马组织中的铜、锌含量及其比值均较低,差异均有统计学意义(P0.05);且随着纳米硫化铅染毒剂量的升高,大鼠的逃避潜伏期和首次到达平台时间均呈延长趋势,停留目的象限时间呈缩短趋势,穿越平台次数呈减少趋势,全血和海马组织中的铅含量均呈上升趋势,海马组织中的铜、锌含量及其比值均呈下降趋势。结论纳米硫化铅可致大鼠学习记忆能力降低,并引起海马组织中铜锌含量、铜/锌比值的下降。
[Abstract]:Objective to investigate the effects of nano-lead sulfide exposure on learning and memory ability and the contents of copper and zinc in hippocampus of rats. Methods 24 healthy adult SD rats of SPF grade were randomly divided into 3 groups. 8 rats in each group were exposed to sodium sulphide for 15 ~ 30 mg/kg, respectively. The volume of exposure was 5 ml / kg, once a week, by trachea injection, and the volume of exposure was 5 ml / kg, once a week, and the volume of exposure was 5 ml 路kg ~ (-1) 路kg ~ (-1), respectively. Morris water maze test was used to test the learning and memory ability of rats. The contents of lead in whole blood and hippocampus, copper and zinc in hippocampus and their ratios were measured by Morris water maze test. The results were compared with those in control group. The escape latency and the first arrival time were prolonged, the duration of stay in the quadrant was shortened, the number of crossing the platform was decreased, the lead content in the whole blood and hippocampus was higher, and the copper content in the hippocampus was higher than that in the control group. The zinc content and its ratio were lower, the difference was statistically significant (P 0.05), and with the increase of the dose of lead sulfide, the escape latency and the first arrival time of the rats showed a tendency of prolonging, while the time of staying in the target quadrant showed a tendency of shortening. The times of crossing the platform decreased, the lead content in whole blood and hippocampus increased, and the copper, zinc and its ratio in hippocampus decreased. Conclusion the learning and memory ability of rats can be decreased by nanometer lead sulfide. The content of Cu and Zn and the ratio of Cu / Zn in hippocampus were decreased.
【作者单位】：华北理工大学公共卫生学院;北京军区北戴河疗养院;邢台市第三医院医院感染科;开滦总医院神经内科;
【分类号】：R114

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