桑葚浓缩汁对D-半乳糖诱导氧化损伤大鼠肾脏的保护作用

发布时间：2018-04-02 20:09

本文选题：桑葚浓缩汁　切入点：D-半乳糖　出处：《食品科学》2017年23期

【摘要】：研究桑葚浓缩汁对氧化损伤大鼠肾脏的保护作用。采用D-半乳糖(D-galactose,D-Gal)建立衰老模型,将实验大鼠随机分为6组:空白对照组、阴性对照组(D-Gal模型)、阳性对照组(D-Gal+VE)、桑葚浓缩汁低、中、高剂量组。通过检测大鼠肾脏组织匀浆中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、丙二醛(malondialdehyde,MDA)含量,测定大鼠血清一氧化氮(nitric oxide,NO)、血尿素氮(blood urea nitrogen,BUN)和血清肌酐(serum creatinine,Scr)浓度、总抗氧化能力(total antioxidant capacity,T-AOC)及B淋巴细胞瘤-2相关X蛋白(B-cell lymphoma-2-associated X protein,Bax)基因的表达,并通过病理组织学切片评价桑葚浓缩汁对氧化损伤蛋白的影响。结果表明:与阴性对照组相比,桑葚浓缩汁中、高剂量组可以显著提高肾组织SOD、GSH-Px、CAT活力和T-Ao C(P0.05);降低MDA、BUN及Scr浓度;抑制NO浓度升高(P0.05),并成一定量效依赖关系;Bax表达降低;其中桑葚浓缩汁高剂量效果最好,GSH-Px活力为150.33 U、Scr浓度为34.54μmol/L、BUN浓度为8.96 mmol/L,均达到了阳性对照组水平(P0.05);NO浓度为29.78μmol/L,达到空白对照组水平(P0.05)。病理学组织切片显示,与阴性对照组相比,桑葚处理组症状明显减轻,尤其中、高剂量组,极少量肾小管上皮细胞胞浆疏松、淡染,无明显的充血、出血。综上所述,桑葚浓缩汁对D-Gal诱导氧化损伤大鼠肾脏有较好的保护作用。
[Abstract]:To study the protective effect of concentrated mulberry juice on the kidney of oxidative injury rats.The aging model was established by using D-galactoseto D-Galal. The experimental rats were randomly divided into six groups: blank control group, negative control group, D-Gal model group, positive control group, low, middle and high dose mulberry juice concentrate group.The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (malondialdehyde) (MDAs) in rat kidney homogenate were measured.The levels of serum nitric oxide (urea) and creatinine creatinine (Scrs), total antioxidant capacityT-AOC() and B-cell lymphoma-2-associated X protein (Bax) gene expression in serum of rats were measured.The effect of concentrated mulberry juice on oxidative damage protein was evaluated by histopathological section.Histopathological sections showed that compared with the negative control group, the symptoms of mulberry treatment group were significantly alleviated, especially in the high dose group, a very small number of renal tubular epithelial cells were loose, light stained, no obvious congestion, bleeding.In conclusion, Mulberry concentrate has a good protective effect on the kidney of D-Gal induced oxidative injury in rats.
【作者单位】：鲁东大学食品工程学院;山东商务职业学院食品工程系;
【基金】：国家自然科学基金青年科学基金项目(31401491) 城新创新奖学金项目大学生创新创业训练计划项目(7422)
【分类号】：R151

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