乙酸铅及硫酸铝和甲醛暴露对大鼠不同脑区血脑屏障通透性的影响

发布时间：2018-04-04 07:21

本文选题：乙酸铅　切入点：硫酸铝　出处：《环境与健康杂志》2015年03期

【摘要】：目的定量评估乙酸铅、硫酸铝和甲醛暴露对大鼠不同脑区血脑屏障(blood-brain barrier,BBB)通透性的影响。方法设低(1/8 LD50)、中(1/4 LD50)、高剂量(1/2 LD50)和空白对照组(不作处理),采用腹腔注射染毒Wistar大鼠。于染毒后24、48和72 h采用伊文蓝(EB)比色法测定大鼠大脑皮质、皮质下及海马组织血脑屏障通透性。血脑屏障通透性以每毫克组织中EB的光密度值表示(OD/mg)。结果与对照组比较,低、中、高剂量乙酸铅(18.75、37.5和75.0 mg/kg)、硫酸铝(7.5、15.0和30.0 mg/kg)染毒后24、48和72 h大鼠大脑皮质、皮质下及海马组织血脑屏障通透性增高(P0.01)。各剂量甲醛染毒组(50.0、100.0和200.0 mg/kg)大鼠皮质下及海马组织血脑屏障通透性在所有观察时点均增高(P0.05),脑皮质血脑屏障通透性在第48和72小时增高(P0.05)。结论乙酸铅、硫酸铝和甲醛暴露能够引起大鼠大脑皮质、皮质下及海马组织的血脑屏障通透性增高,提示对上述脑区血脑屏障功能具有损伤性,较易侵入大脑甚至对中枢神经产生暴露和影响。
[Abstract]:Objective to evaluate quantitatively the effects of lead acetate, aluminum sulfate and formaldehyde on the permeability of blood-brain barrier (BBB) in different brain regions of rats.Methods Wistar rats were injected intraperitoneally with 1 / 8 LD50, 1 / 4 LD50, 1 / 2 LD50 and 1 / 2 LD50, respectively, and the control group (untreated) was injected intraperitoneally with Wistar rats.The permeability of blood-brain barrier in rat cerebral cortex, subcortical and hippocampal tissues was measured by Yi Wen blue EB-colorimetric method at 24 hours, 48 and 72 hours after exposure.Blood-brain barrier permeability is expressed as the optical density of EB per milligram of tissue.Results compared with the control group, the blood brain barrier permeability of rats exposed to high doses of lead acetate 18.75 mg / kg and 75.0 mg / kg, aluminum sulfate 7.5% 15.0 and 30.0 mg / kg at 24 h, 48 h and 72 h after exposure increased the permeability of the blood-brain barrier in the rat cerebral cortex, subcortical and hippocampal tissues (P0.01).The blood-brain barrier permeability of rat subcortical and hippocampal tissues increased at all observation points (P 0.05), and the permeability of cerebral cortex blood-brain barrier increased at 48 h and 72 h (P 0.05) in rats exposed to formaldehyde at each dose of 50.0100.0 and 200.0 mg 路kg ~ (-1) 路L ~ (-1) 路kg ~ (-1) 路kg ~ (-1).Conclusion lead acetate, aluminum sulfate and formaldehyde exposure can increase the permeability of blood-brain barrier in cerebral cortex, subcortical and hippocampal tissues of rats.It is easy to invade the brain and even to the central nervous system exposure and influence.
【作者单位】：中国疾病预防控制中心环境与健康相关产品安全所环境毒理室;
【基金】：国家自然科学基金(81072260)
【分类号】：R114

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