1,2-二甲基-3-羟基-4-吡啶酮和牛磺酸联合干预对铝染毒大鼠大脑皮层抗氧化系统的保护作用

发布时间：2018-04-05 13:34

  本文选题：铝　切入点：-二甲基--羟基--吡啶酮　出处：《环境与健康杂志》2015年11期

【摘要】：目的探讨1,2-二甲基-3-羟基-4-吡啶酮(deferipone,DFP)和牛磺酸联合作用对染铝大鼠大脑皮层抗氧化系统的影响。方法将70只健康SPF级雄性Wistar大鼠按体重随机分为7组,以灌胃方式染毒,其中,阴性对照组给予1 ml生理盐水,连续8周;铝染毒组、牛磺酸干预组、低剂量DFP干预组、高剂量DFP干预组、牛磺酸+低剂量DFP干预组和牛磺酸+高剂量DFP干预组在前4周分别给予281.40 mg/kg三氯化铝溶液,后4周分别给予1 ml生理盐水、400 mg/kg牛磺酸、13.82 mg/kg DFP、27.44 mg/kg DFP、400 mg/kg牛磺酸、400 mg/kg牛磺酸;牛磺酸+低剂量DFP干预组和牛磺酸+高剂量DFP干预组给予400 mg/kg牛磺酸6 h后分别给予13.82、27.44 mg/kg DFP,每天1次。测定大鼠大脑皮层SOD、GSH-Px活力和MDA含量。结果与阴性对照组比较,铝染毒组大鼠大脑皮层SOD和GSH-Px活力均较低,而MDA含量较高,差异有统计学意义(P0.05)。与铝染毒组相比,在单独干预组中,仅高剂量DFP干预组可以显著提高SOD和GSH-Px活力,降低MDA的含量;牛磺酸干预组可以显著提高GSH-Px的活力水平以及降低MDA含量。在DFP和牛磺酸的联合作用下,大鼠大脑皮层的SOD和GSH-Px活力水平以及MDA含量均基本恢复正常。其中,与牛磺酸干预组和低剂量DFP干预组比较,牛磺酸+DFP干预组可以显著提高SOD和GSH-Px活力,降低MDA含量;且以牛磺酸+低剂量DFP干预组尤为显著。结论在本实验剂量范围内,牛磺酸和DFP联合干预可以对染铝大鼠大脑皮层的抗氧化系统起到保护作用,且效果优于牛磺酸或DFP单独干预。
[Abstract]:Objective to investigate the effects of 1-dimethyl-3-hydroxy-4-pyridine-deferipone (DFPFP) and taurine on the antioxidation system in the cerebral cortex of rats exposed to aluminum.Methods 70 healthy SPF grade male Wistar rats were randomly divided into 7 groups according to their body weight. Among them, the negative control group was given 1 ml of normal saline for 8 weeks, the aluminum group, taurine intervention group, and low dose DFP intervention group.High dose DFP intervention group, taurine low dose DFP intervention group and taurine high dose DFP intervention group were given 281.40 mg/kg aluminum trichloride solution in the first 4 weeks, respectively.At the end of 4 weeks, 1 ml normal saline for 400 mg/kg taurine was administrated with 13.82 mg/kg DFPX 27.44 mg/kg mg/kg taurine and 400 mg/kg taurine respectively.Taurine low dose DFP intervention group and taurine high dose DFP intervention group were treated with taurine for 6 h, 13.82 mg/kg 27.44 mg/kg DFP, once a day, respectively.The activity of GSH-Px and the content of MDA in cerebral cortex of rats were measured.Results compared with the negative control group, the activity of SOD and GSH-Px in the cerebral cortex of rats exposed to aluminum was lower, while the content of MDA was higher, the difference was statistically significant (P 0.05).Compared with aluminum exposure group, only high dose DFP group could significantly increase the activity of SOD and GSH-Px and decrease the content of MDA, while taurine group could significantly increase the level of GSH-Px activity and decrease the content of MDA.Under the combined action of DFP and taurine, the activities of SOD and GSH-Px and the content of MDA in the cerebral cortex of rats returned to normal.Compared with taurine intervention group and low dose DFP intervention group, taurine DFP intervention group could significantly increase SOD and GSH-Px activity and decrease MDA content, especially in taurine low dose DFP intervention group.Conclusion the combined intervention of taurine and DFP can protect the antioxidation system in the cerebral cortex of aluminized rats, and the effect is better than that of taurine or DFP alone.
【作者单位】： 山东大学公共卫生学院卫生检验学系;
【基金】：山东省自然科学基金(ZR2013CM043)
【分类号】：R114
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本文编号：1714978