氟铝联合持续暴露对二代仔鼠神经生理发育及海马结构的影响

发布时间：2018-04-08 22:19

本文选题：氟　切入点：铝　出处：《现代预防医学》2017年09期

【摘要】：目的探讨氟铝联合持续暴露对二代仔鼠神经生理发育和海马结构的影响。方法 SD孕鼠36只,随机分为9组(每组4只):对照组和单独氟化钠(100、200 mg/L)、三氯化铝(500、1 000 mg/L)染毒组及氟化钠+三氯化铝联合染毒组。从母鼠妊娠第0天至二代仔鼠出生第60天(PND60),自由饮水染毒。每组一代仔鼠于PND90(性成熟)随机选取6只(雌雄比为2∶1)合笼。每组二代仔鼠出生后,随机选取8只(雌雄各半),观察神经生理发育指标;PND60,收集12 h尿液,处死后留取大脑,离子选择电极法、电感耦合等离子体发射光谱法分别测定尿和脑中氟、铝;HE染色观察海马变化。结果与对照组比较,各染毒组脑重系数、开眼和出牙未见差异;高氟组、低氟低铝组和高氟低铝组平面翻正反射时间延长,高剂量氟、铝及氟铝联合组二代仔鼠悬崖回避反射时间延长(P0.05)。HE染色显示高剂量氟、铝及氟铝联合组CA3区出现病理改变。结论高剂量氟、铝及氟铝联合可能致二代仔鼠的神经生理发育及海马损伤。
[Abstract]:Objective to investigate the effects of combined fluoride and aluminum exposure on neurophysiological development and hippocampal formation in second generation rats.Methods Thirty-six pregnant SD rats were randomly divided into 9 groups (4 rats in each group: control group, 100 200 mg / L sodium fluoride alone group, 1 000 mg / L aluminum chloride 500 mg / L group) and sodium fluoride trichloride combined group.From the first day of pregnancy to the 60th day of birth of the second generation, the mice were exposed to the free drinking water.Six offspring (2: 1 female / male) were randomly selected from each group in PND 90 (sexual maturity).Eight rats (half male and half female) were randomly selected from each group after birth. The neurophysiological development index (PND60) was observed. Urine samples were collected for 12 hours. The brain was collected after death and the brain was retained by ion selective electrode method.Inductively coupled plasma emission spectrometry (ICP-AES) was used to detect fluoride in urine and brain respectively.Results compared with the control group, there was no difference in brain weight coefficient, open eye and teeth in each exposure group, and the time of positive reflex was prolonged in the high fluorine group, low fluoride low aluminum group and high fluorine low aluminum group, and high dose fluoride.The cliff avoidance reflex time of the second generation rats in the combined group of aluminum and fluorine was prolonged (P 0.05). He staining showed high dose of fluorine, and the pathological changes in the CA3 region of the group of aluminum and fluoro aluminum.Conclusion the combination of high dose fluoride, aluminum and aluminum fluoride may induce neurophysiological development and hippocampal injury in second generation rats.
【作者单位】：贵州医科大学公共卫生学院环境卫生学教研室;毕节医学高等专科学校;贵州省黔东南州疾病预防控制中心;
【基金】：国家自然科学基金地区基金资助(81560519)
【分类号】：R114

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