亮氨酸对高脂高胆固醇喂养小鼠瘦素抵抗影响

发布时间：2018-04-10 14:13

本文选题：亮氨酸 + 瘦素抵抗　；参考：《中国公共卫生》2017年05期

【摘要】：目的探讨亮氨酸对高脂高胆固醇喂养小鼠体脂和瘦素抵抗的影响。方法 60只雄性C57BL/6J小鼠随机分为对照组、高脂高胆固醇组、低、高剂量亮氨酸组(1.5%、3.0%亮氨酸),干预24周;测定小鼠体重、脂肪重量、血清胆固醇和瘦素水平,Western blot法检测小鼠睾周脂肪组织中瘦素受体(Ob-R)、Janus蛋白酪氨酸激酶2(JAK2)、信号转导和转录激活子3(STAT3)及细胞因子信号转导抑制因子3(SOCS3)的蛋白表达。结果高脂高胆固醇喂养明显升高小鼠体重、脂重和血清胆固醇水平;与高脂高胆固醇组比较,亮氨酸组小鼠体重、脂重和血清胆固醇水平明显下降;与对照组比较,高脂高胆固醇组小鼠血清瘦素水平明显升高[(7.14±2.44)ng/mL,](P0.01);与高脂高胆固醇组比较,1.5%、3.0%亮氨酸组小鼠瘦素水平[分别为(4.21±2.37)、(4.24±2.22)ng/mL]明显降低(P0.01);与对照组比较,高脂高胆固醇组小鼠睾周脂肪组织中Ob-R蛋白表达下调;与高脂高胆固醇组比较,亮氨酸组小鼠睾周脂肪组织中Ob-R、JAK2蛋白表达上调、SOCS3蛋白表达下调。结论亮氨酸干预可抑制高脂高胆固醇喂养引起的小鼠体重和体脂增加,其机制可能与调节瘦素受体及其下游蛋白JAK2和SOCS3表达、改善瘦素抵抗有关。
[Abstract]:Objective to investigate the effect of leucine on body fat and leptin resistance in mice fed with high fat and high cholesterol.Methods 60 male C57BL/6J mice were randomly divided into control group, high cholesterol group, low dose leucine group and high dose leucine group for 24 weeks.The levels of serum cholesterol and leptin were determined by Western blot to detect the protein expression of leptin receptor Ob-Rn Janus protein tyrosine kinase 2JAK2, signal transduction and activator of transcription 3 STAT3 and cytokine signal transduction inhibitor 3SOCS3.Results the body weight, fat weight and serum cholesterol level of mice fed with high fat and high cholesterol were significantly increased, compared with those of high fat and high cholesterol group, the body weight, fat weight and serum cholesterol level of leucine group were significantly lower than those of control group.The expression of Ob-R protein was down-regulated in the peri-testis adipose tissue of mice with high lipid and high cholesterol, and the expression of Ob-R- ~ (-) JAK2 protein was down-regulated in the peri-testis adipose tissue of leucine group as compared with that in high-lipid and high-cholesterol group.Conclusion Leucine intervention can inhibit the increase of body weight and body fat induced by high fat and high cholesterol feeding in mice. The mechanism may be related to the regulation of the expression of leptin receptor and its downstream protein JAK2 and SOCS3 and the improvement of leptin resistance.
【作者单位】：苏州大学公共卫生学院营养与食品卫生教研室;苏州大学放射医学与防护学院;
【基金】：国家自然科学基金(81472974;81302425;81273067) 江苏省高校“青蓝工程”中青年学术带头人培养对象
【分类号】：R151

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