苹果叶总黄酮的提取及其含量测定

发布时间：2018-04-11 09:01

  本文选题：苹果叶 + 总黄酮　； 参考：《北方园艺》2015年14期

【摘要】：以"红元帅"苹果的干燥叶子为试材,通过单因素试验确定苹果叶总黄酮的最佳提取工艺条件,采用可见分光光度法对苹果叶中总黄酮含量进行测定,以期为苹果叶中总黄酮提取工艺及含量测定提供方法和依据。结果表明:苹果叶总黄酮的最佳提取工艺条件为乙醇作溶剂,乙醇浓度60%,料液比1∶20g/mL,提取温度为70℃,提取时间为30min;在选定试验条件下对苹果叶进行含量测定,对芦丁对照品浓度与吸光度进行线性回归,其回归方程为Y=24.821 43X-0.031,R2=0.999 45(n=6),芦丁对照品质量在4~24μg范围内呈良好的线性关系,在此条件下测定苹果叶中总黄酮平均含量为30.28mg/g。试验优选出了苹果叶中总黄酮的最佳提取工艺条件及含量测定方法,该方法简单、快速,准确,精密度,稳定性,重现性良好。
[Abstract]:Using dried leaves of "Red Marshal" apple as test material, the optimum extraction conditions of total flavonoids from apple leaves were determined by single factor test, and the content of total flavonoids in apple leaves was determined by visible spectrophotometry.In order to provide a method and basis for the extraction and determination of total flavonoids in apple leaves.The results showed that the optimum conditions of extracting total flavonoids from apple leaves were ethanol as solvent, ethanol concentration as 60%, ratio of material to liquid 1: 20 g / mL, extraction temperature 70 鈩，

本文编号：1735328