大气细颗粒物对人支气管上皮细胞的活性抑制和炎性作用

发布时间：2018-04-11 12:00

本文选题：大气细颗粒物 + 人支气管上皮细胞　；参考：《中南大学学报(医学版)》2017年09期

【摘要】：目的:研究大气细颗粒物(fine particulate matter,PM2.5)对人支气管上皮细胞活性的影响及其炎性作用。方法:用PM2.5采样器采集上海地区大气PM2.5样本,扫描电镜观察PM2.5形态特征。将人支气管上皮细胞BEAS-2B暴露于不同浓度(0,50,100,200,400,800μg/m L)的PM2.5 12,24,48 h,细胞活力检测试剂盒(cel l counting kit-8,CCK-8)法检测PM2.5暴露对细胞活性的影响。实时定量PCR(quantitative real-time PCR,q RT-PCR)检测细胞粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)和TNF-αm RNA的表达,Western印迹检测GM-CSF和TNF-α蛋白的表达。结果:扫描电镜观察发现,PM2.5形态多样,大小不一,直径大多等于或小于2.5μm。与同时间点未暴露组比较,各暴露组(50~800μg/m L)细胞活性呈不同程度的下降,差异具有统计学意义(P0.05)。与未暴露组比较,暴露于100,400或800μg/m L PM2.5 24 h后,GM-CSF和TNF-αm RNA和蛋白表达水平明显升高(P0.05),且PM2.5暴露浓度越高,GM-CSF和TNF-α的m RNA和蛋白升高水平越显著。结论:大气PM2.5可引起人支气管上皮细胞的炎症反应,降低细胞活性,这可能与PM2.5促发和加重支气管肺部炎性疾病有关。
[Abstract]:Objective: To study the atmospheric fine particles (fine particulate, matter, PM2.5) effect on the activity of human bronchial epithelial cells and inflammatory effects. Methods: collected in the atmosphere of Shanghai PM2.5 samples with PM2.5 sampler, the morphology of PM2.5 was observed by scanning electron microscopy. The characteristics of human bronchial epithelial cells exposed to different concentrations of BEAS-2B (0,50100200400800 g/m L). PM2.5 12,24,48 h, the cell viability detection kit (cel l counting kit-8 CCK-8) method was used to detect the effects of PM2.5 exposure on cell activity. Real time quantitative PCR (quantitative real-time PCR, Q RT-PCR) cell granulocyte macrophage colony stimulating factor (granulocyte-macrophage colony, stimulating factor, GM-CSF) TNF- and the expression of alpha m RNA the expression of GM-CSF, Western blot and TNF- alpha protein. Results: scanning electron microscope showed that the PM2.5 shape, size and diameter are equal to or less than 2 .5 M. and at the same time point of non exposed group, the exposed group (50~800 g/m L) cell activity was decreased, the difference was statistically significant (P0.05). And the non exposed group, exposure to 100400 or 800 g/m L PM2.5 24 h, the expression level of GM-CSF and TNF- alpha m RNA and the protein increased significantly (P0.05), and PM2.5 exposure and the higher the concentration of M RNA and protein GM-CSF and TNF- alpha level is more significant. Conclusions: the PM2.5 can cause inflammation of bronchial epithelial cells, reduce the cell activity, which may be related to PM2.5 priming and exacerbation of bronchial pulmonary inflammatory diseases.

【作者单位】：厦门大学附属第一医院耳鼻咽喉头颈外科;复旦大学环境科学与工程系;
【分类号】：R12;R56

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