交链孢毒素在Caco-2细胞中的转运与吸收

发布时间：2018-04-12 14:03

本文选题：Caco-细胞 + 模型　；参考：《山东大学学报(医学版)》2015年06期

【摘要】：目的利用构建的结肠腺癌细胞系(Caco-2)细胞模型,探讨交链孢毒素经Caco-2细胞模型的跨膜转运与吸收特征。方法将Caco-2细胞接种于Transwell培养板的聚碳酯膜上培养21 d,用跨上皮细胞电阻(TEER)、碱性磷酸酶(ALP)活性、荧光素表观渗透系数(Papp)和细胞形态学等指标评价所构建的Caco-2细胞模型。通过测定细胞单层顶侧和基底膜侧交链孢毒素的含量,研究不同浓度和转运时间交链孢酚(AOH)、交链孢酚单甲醚(AME)、交链孢菌酮酸(Te A)溶液的转运与吸收。结果培养至第21天的Caco-2细胞其TEER超过(300±15)Ω·cm2,且细胞顶侧和基底膜侧ALP活性差异有统计学意义(P0.01)。透射电镜下可见清晰的微绒毛和细胞间的紧密连接。AOH、AME和Te A的Papp范围为(1~10)×10-5cm/s,不同浓度和转运时间的毒素吸收率范围为1.5%~63.0%。结论建立的模型形成了完整且紧密的细胞单层。AOH和AM E的Papp大小与毒素浓度和转运时间有关,AOH、AME和Te A为易于吸收或中等易吸收的毒素。
[Abstract]:Objective to investigate the transmembrane transport and absorption characteristics of Alternterin through Caco-2 cell model using a colon adenocarcinoma cell line (Caco-2).Methods Caco-2 cells were cultured on the polycarboxylate membrane of Transwell culture plate for 21 days. The Caco-2 cell model was evaluated by transepithelial cell resistance, alkaline phosphatase (ALP) activity, fluorescein apparent osmotic coefficient (Pappa) and cell morphology.The transport and absorption of Alternaria Alternaria in different concentration and transport time were studied by measuring the content of Alternaria arenae in monolayer and basement membrane. The transport and absorption of AMEA, Alternaria ketoacid (Te) in different concentrations and transport time were studied.Results the TEER of Caco-2 cells cultured to 21 days was more than 300 卤15 惟 cm 2, and the difference of ALP activity between the apical side and basal membrane side was statistically significant (P 0.01).Under transmission electron microscope, the Papp range of clear tight junctions between microvilli and cells. The Papp range of AOHAME and Te A was 10 脳 10 ~ (-5) cm / s, and the toxin absorption rate of different concentration and transport time was 1.5 ~ 63.0 cm / s.Conclusion the Papp size of the complete and compact monolayer. AOH and AM E is related to the concentration and transport time of the toxin. AME and Te A are easy to be absorbed or moderately easily absorbed.
【作者单位】：中国疾病预防控制中心营养与健康所;国家食品安全风险评估中心卫生部食品安全风险评估重点实验室;中国疾病预防控制中心病毒病预防控制所;
【基金】：国家高技术研究发展(863)计划(2012AA101603)
【分类号】：R114

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