微孔板和分光光度计法检测7种食用菌的抗氧化活性

发布时间：2018-04-18 02:29

本文选题：食用菌 + 抗氧化活性　；参考：《中成药》2015年12期

【摘要】：目的建立微量检测食用菌体外清除二苯代苦味酰基自由基(DPPH·)活性的方法,并比较常见食用菌的抗氧化活性,为寻找天然抗氧化剂提供初步的筛选方法和技术资料。方法超声提取得到香菇等7种食用菌和火麻仁的提取物后,采用微孔板与分光光度计法测定其DPPH·清除率,然后对这两种方法的测定结果进行曲线拟合,并对实验耗时、试剂用量和方法重复性方面进行对比,进而比较其DPPH·清除活性。结果两种方法所得的DPPH·清除率无统计学差异,但在实验耗时、试剂用量等方面,微孔板法有明显优势。另外,食用菌水提物的抗氧化活性均明显高于醇提物,其中香菇水提物的活性最高。结论微孔板法测定食用菌DPPH·的清除活性时具有准确、快捷、重复性好等特点,而且食用菇水溶性成分的抗氧化活性大于醇溶性成分,可为后续产品的开发提供技术资料。
[Abstract]:Objective to establish a microassay method for scavenging dibenzo-acyl radical DPPH in edible fungi in vitro, and to compare the antioxidant activity of common edible fungi, and to provide a preliminary screening method and technical data for searching for natural antioxidants.Methods the DPPH clearance rate was determined by microporous plate and spectrophotometer. The results of the two methods were fitted by curve, and the experiment time was used.The amount of reagent was compared with the repeatability of the method, and then the DPPH scavenging activity was compared.Results there was no significant difference in the clearance rate of DPPH between the two methods, but the microporous plate method had obvious advantages in terms of time consuming and reagent dosage.In addition, the antioxidant activity of water extract of edible fungi was significantly higher than that of alcohol extract, and the activity of water extract of Lentinus edodes was the highest.Conclusion the micropore plate method is accurate, rapid and reproducible in determining the scavenging activity of edible mushroom DPPH, and the antioxidant activity of water-soluble components of edible mushroom is higher than that of alcohol-soluble component, which can provide technical data for the further development of edible mushroom.
【作者单位】：河南中医学院药学院;
【基金】：国家自然科学基金面上项目(81473435) 河南省高等学校青年骨干教师资助计划项目(2013GGJS-092) 河南省教育厅科学技术重点资助项目(14A310023) 河南中医学院研究生创新基金项目(2014YCX009);河南中医学院药学院大学生创新学习项目(YXCX[2014]22)
【分类号】：R155.5

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