气相色谱-质谱法同时分析运动营养品中的大麻酚、大麻二酚和Δ9-四氢大麻酚

发布时间：2018-04-18 18:39

本文选题：大麻酚 + 大麻二酚　；参考：《中国运动医学杂志》2015年04期

【摘要】：目的:建立气相色谱-质谱法同时分析运动营养品中大麻酚、大麻二酚和Δ9-四氢大麻酚的检测方法。方法:样品采用液液萃取,提取液经过正相固相萃取柱(Silica)纯化,洗脱液氮气吹干,N-甲基-N-(三甲基硅烷)三氟乙酰胺衍生化后,采用HP-1MS柱(17 m×0.2 mm i.d.×0.11 mm)色谱分离,程序升温,质谱检测,D3-Δ9-四氢大麻酚为内标。结果:大麻酚、大麻二酚和Δ9-四氢大麻酚的衍生化产物实现基线分离,三个定性特征离子的峰度比计算结果可以满足世界反兴奋剂机构(WADA)要求。该方法的检测限为1μg/kg,定量限为2μg/kg,在添加的高、中、低三种浓度的相对回收率分布在94%~119%之间。结论:该方法满足常规检测要求,样品前处理简单、快速、可靠。
[Abstract]:Objective: to establish a gas chromatography-mass spectrometry method for simultaneous determination of cannabinol, cannabinol and 螖 9 tetrahydrocannabinol in sports nutrients.Methods: the sample was extracted by liquid-liquid extraction, the extract was purified by normal phase solid phase extraction column (Silica), and the eluate was dried by nitrogen. After derivatization of N-methyl-N-trifluoroacetamide, the chromatographic separation was performed on a HP-1MS column of 17 m 脳 0.2 mm i.d. 脳 0.11 mm.D3-螖 9-tetrahydrocannabinol was identified as internal standard by mass spectrometry.Results: the derivatization products of cannabinol, cannabinol and 螖 9-tetrahydrocannabinol were separated from baseline. The results of kurtosis ratio of the three qualitative characteristic ions could meet the requirements of the World Anti-Doping Agency (WADAA).The detection limit of the method is 1 渭 g / kg and the quantitative limit is 2 渭 g / kg. The relative recoveries of the three concentrations of high, middle and low concentrations are between 94g / kg and 119%.Conclusion: this method can meet the requirements of routine detection, and the sample pretreatment is simple, rapid and reliable.
【作者单位】：国家体育总局反兴奋剂中心食品药品兴奋剂检测实验室;
【分类号】：R155.5;O657.63

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