西兰花茎叶多肽的酶法提取及其增强免疫力功能研究

发布时间：2018-04-20 09:34

本文选题：西兰花 + 多肽　；参考：《食品工业科技》2017年11期

【摘要】：本文对西兰花茎叶多肽进行酶法提取以筛选增强免疫力功能活性强的组分。从西兰花茎叶中提取粗蛋白后,首先经酶法制备西兰花多肽,然后采用Valflow50超滤、Sephadex G-15进行分离纯化,得到分子量为310~2600 Da的西兰花小肽,并对其进行增强免疫力功能的动物实验研究,分别以100、50、25 mg/kg·bw 3个剂量的西兰花多肽提取物对小鼠连续灌胃30 d,然后分别测定小鼠的体液免疫功能、细胞免疫功能和单核-巨噬细胞功能。结果表明,高剂量组(100 mg/kg)西兰花多肽提取物对小鼠的淋巴细胞转化能力(0.225±0.040)与小鼠的左右耳片重量差异(18.19±2.94)mg均显著高于空白对照组(p0.05)。西兰花多肽提取物对正常小鼠碳粒廓清功能有非常明显的促进作用,碳廓清指数分别为(0.126±0.052)和(0.136±0.022),高、中剂量能显著(p0.05)增加小鼠非特异免疫功能。以上结果表明西兰花多肽提取物具有增强免疫力的功能,此研究可为西兰花的深度开发和综合利用提供基础数据和科学参考。
[Abstract]:In this paper, broccoli stem and leaf polypeptides were extracted by enzymatic method to screen the components with strong immune function. After extracting crude protein from the stems and leaves of broccoli, broccoli peptides were prepared by enzymatic method, and then purified by Valflow50 ultrafiltration Sephadex G-15. The small peptides of broccoli with molecular weight of 310 ~ 2600Da were obtained. The mice were perfused with three doses of Broccoli polypeptide extract of 100m 50g 25 mg/kg BW for 30 days, then the humoral immune function of mice was measured. Cellular immune function and monocyte-macrophage function. The results showed that the lymphocyte transformation ability of high dose group was significantly higher than that of control group (0.225 卤0.040) and the weight difference of left and right ear slices of mice (18.19 卤2.94)mg) than that of control group (P 0.05). The polypeptide extract of broccoli significantly promoted the clearance of carbon particles in normal mice. The carbon clearance index was 0.126 卤0.052) and 0.136 卤0.022, respectively. The medium dose could significantly increase the non-specific immune function of mice. The above results indicate that the extract of broccoli polypeptide has the function of enhancing immunity. This study can provide basic data and scientific reference for the further development and comprehensive utilization of broccoli.
【作者单位】：浙江省医学科学院;
【基金】：浙江省重大科技计划项目(2014C02023) 浙江省医药卫生项目(201473497)
【分类号】：R151;TQ464.7

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