锰暴露对雄性大鼠肝功能酶谱影响的时间毒性

发布时间：2018-04-21 06:39

本文选题：时间毒性 + 丙氨酸转氨酶(ALT)　；参考：《现代预防医学》2017年11期

【摘要】：目的研究不同时间点的锰暴露对雄性大鼠肝功酶活性的影响,为锰的毒性评价与防治提供科学依据。方法健康成年雄性SD大鼠96只,在严格控制光照/黑暗(12h:12h)条件下适应性喂养7天后,随机分为4个对照组与4个锰暴露组,分别于一天中的ZT2、ZT8、ZT14与ZT20授时时间点对锰暴露组腹腔注射30mg/kg Mn Cl2,对照组等剂量腹腔注射蒸馏水,每天1次,连续21天。第22天,处死动物,获取动物血清,检测其丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)及酸性磷酸酶(ACP)活性。分析各指标的组间差异及昼夜节律。结果锰暴露可引起雄性大鼠血清肝功酶活性的改变,且不同的时间点暴露,结果却不相一致。在授时时间点ZT2暴露其酶谱改变较相一致,而其他的授时时间点暴露特别是ZT8其酶谱改变差异较大。余弦分析结果显示ALT、AST、ACP与ALP日均值均下降,ALT与AST振幅减少、ACP与ALP振幅增加,ALT与ACP的峰值时间延迟、而AST与ALP的峰值时间提前。其中ALP对照组具昼夜节律特性(P0.05),而锰暴露后昼夜节律消失(P0.05)。结论锰暴露引起的血清肝功酶活性改变具时间差异性,且可导致其生物节律紊乱;在安排锰暴露工人的工作时间时,需尽可能地避开毒性最大的时段。在进行锰肝毒性评价时,需尽可能地安排在毒性反应相一致的时间点进行。
[Abstract]:Objective to study the effects of manganese exposure at different time points on hepatic enzyme activity in male rats and to provide scientific basis for the evaluation and prevention of manganese toxicity. Methods Ninety-six adult male SD rats were randomly divided into 4 control groups and 4 manganese exposure groups after 7 days of adaptive feeding under strictly controlled light / dark light (12 h: 12 h). 30mg/kg mn Cl _ 2 was injected intraperitoneally into the manganese exposure group at the time point of ZT2 + ZT8 + ZT14 and ZT20 in one day, and distilled water was injected intraperitoneally in the control group at the same dose, once a day for 21 days. On the 22nd day, the animals were killed and their serum was obtained. The activities of alanine aminotransferase (alt), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and acid phosphatase (ACP) were detected. The differences and circadian rhythm of each index were analyzed. Results Manganese exposure induced the changes of serum liver function enzyme activity in male rats, and the results were not consistent at different time points. The changes of zymogram of ZT2 exposed to time point were consistent, but that of other time points, especially ZT8, varied greatly. The results of cosine analysis showed that the daily mean values of alt and ALP both decreased and the amplitudes of alt and AST decreased. The amplitudes of ACP and ALP increased and the peak time of alt and ACP was delayed, while the peak time of AST and ALP was earlier. In ALP control group, the circadian rhythm was P0.05, but the circadian rhythm disappeared after manganese exposure. Conclusion the changes of serum liver enzyme activity caused by manganese exposure have time difference and may lead to the disorder of biological rhythm. When arranging the working hours of workers exposed to manganese, the period of maximum toxicity should be avoided as far as possible. Manganese hepatotoxicity evaluation should be arranged at the same time as possible.
【作者单位】：桂林医学院公共卫生学院;广西平南第二人民医院检验科;
【基金】：广西自然科学基金项目(2015GXNSFAA139181) 广西高校科研项目(KY2014049)
【分类号】：R114

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