辛基酚对5-羟色胺转运及受体通路的影响

发布时间：2018-04-22 09:02

本文选题：辛基酚 + -羟色胺　；参考：《中国现代医学杂志》2015年14期

【摘要】：目的通过观察人肝7702细胞暴露于辛基酚(OP)后5-羟色胺(5-HT)转运体SERT、5-HT3b受体表达以及5-HT水平的变化,探讨OP对5-HT转运通路和受体结合通路的影响。方法实验分5个组,分别为OP高(5.0μg/L)、中(1.0μg/L)、低(0.2μg/L)剂量组、盐酸氟西汀组和空白对照组,体外细胞培养48 h,采用ELISA试剂盒检测细胞上清液中5-HT含量的变化,运用实时荧光定量PCR法检测转运体SERT及5-HT3b受体的表达。结果中、高剂量组细胞上清液中5-HT含量高于空白对照组(P0.05),细胞中5-HT3b受体的表达降低(P0.05);低、中、高剂量组细胞中SERT的表达均低于空白对照组(P0.05);与盐酸氟西汀组检测结果一致。结论 OP可引起5-HT再摄取障碍,同时减少5-HT3b受体的表达,对机体5-HT代谢网络的转运通路及受体结合通路产生毒效应作用。
[Abstract]:Objective to investigate the effect of OP on 5-HT transport pathway and receptor binding pathway by observing the changes of 5- serotonin (5-HT) transporter SERT, 5-HT3b receptor expression and 5-HT level after exposure to octyl phenol (OP) in human liver 7702 cells. Methods 5 groups were divided into OP high (5 mu g/L), medium (1 mu g/L), low (0.2 micron) dose group, and fluoxetine hydrochloride group. In the blank control group, the cell culture was 48 h in vitro, and the change of 5-HT content in the cell supernatant was detected by ELISA kit. The expression of SERT and 5-HT3b receptor in the transporter was detected by real time fluorescence quantitative PCR method. The results showed that the content of 5-HT in the supernatant of high dose group was higher than that of the blank control group (P0.05), and the expression of 5-HT3b receptor in the cell was reduced (P0.05). The expression of SERT in the low, middle and high dose groups was lower than that in the blank control group (P0.05), which was consistent with the test results of the fluoxetine hydrochloride group. Conclusion OP can cause the 5-HT reuptake barrier and reduce the expression of 5-HT3b receptor, which has a toxic effect on the transport pathway of 5-HT metabolic network and the receptor junction pathway.

【作者单位】：华南农业大学食品学院(广东省食品质量安全重点实验室);
【基金】：广东省自然科学基金(No:S2011010001045) 广东省科技计划项目(No:2012B090600005) 农业部农产品质量安全监管项目(No:GTFP2014011)
【分类号】：R114

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