转基因玉米对大鼠肠道菌群的影响研究

发布时间：2018-04-23 04:25

本文选题：转基因玉米 + 大鼠　；参考：《毒理学杂志》2015年05期

【摘要】：目的观察食用转基因玉米对SD大鼠肠道常见菌群的影响。方法将断乳的SD大鼠随机分为4组:转基因玉米1组(T1)、转基因玉米2组(T2)、非转基因玉米组(N)和阴性对照组(C)。T1和T2组饲料中分别含76.1%和76.4%的转基因玉米,N组饲料中含74.9%的非转基因玉米。喂养大鼠70 d,每周称大鼠体重1次,每周称2次进食量。收集实验开始前后的粪便样品并检测肠道菌群。结果与C组相比,T1、T2和N组进食量均增加,但各组体重及体重增值与C组相比均无统计学意义。T1、T2组与C组和N组相比肠球菌、双歧杆菌、乳杆菌、肠球菌、类杆菌、梭菌和酵母样真菌数均无统计学意义,T2组喂饲前肠杆菌比C组低,喂饲后比喂饲前升高(P0.05),但各组间比较均无统计学意义。结论用转基因玉米喂养SD大鼠70 d未发现其对肠道菌群组成有显著影响。
[Abstract]:Objective to observe the effect of transgenic corn on intestinal flora of SD rats. Methods SD rats were randomly divided into 4 groups: transgenic maize group 1, transgenic maize group 2, non-transgenic corn group N) and negative control group Con. T1 and T2 groups containing 76. 1% and 76. 4% of transgenic corn. It contains 74.9% of non-transgenic corn. Rats were fed for 70 days, weight was given once a week, and food intake was given twice a week. Fecal samples were collected before and after the beginning of the experiment and intestinal flora was detected. Results compared with group C, group T _ 1T _ 2 and group N increased the amount of food intake, but the body weight and body weight increment in each group were not significantly different from those in group C. Group T _ 1T _ 2 compared with group C and group N were compared with group C and group N, and enterococci, bifidobacterium, lactobacillus, enterococci, bacillus spp. There was no significant difference in the number of Clostridium and yeast-like fungi in T2 group, which was lower than that in C group, and increased in P0.05 after feeding, but there was no significant difference between the two groups. Conclusion feeding SD rats with transgenic maize for 70 days has no significant effect on the composition of intestinal flora.
【作者单位】：首都医科大学公共卫生学院;首都医科大学右安门临床检验中心;中国疾病预防控制中心营养与健康所;
【基金】：转基因新品种培育重大专项课题(2014ZX08011-005) 北京市属高等学校高层次人才引进与培养计划项目(CIT&TCD201304177) 首都医科大学“中青年骨干教师”国内交流培养项目
【分类号】：R151

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