纳米硫化镉对A549细胞的损伤研究

发布时间：2018-04-23 14:22

本文选题：纳米硫化镉 + Nano-CdS　；参考：《生态毒理学报》2016年05期

【摘要】：研究纳米硫化镉(Nano-Cd S)材料对肺癌细胞系A549的毒性及氧化损伤作用。培养A549细胞,经传代后接种于6孔板中,每孔2 m L完全培养基,接种次日进行染毒。用直径20~30 nm、长度80~100 nm的Nano-Cd S进行染毒,染毒浓度分别为0、5、10、20、40和80 mg·L~(-1)。染毒24 h后用MTT检测细胞存活率,以存活率在80%左右的浓度为后续实验染毒浓度。应用流式细胞技术,用荧光探针法检测A549细胞的活性氧(reactive oxygen species,ROS)含量,PI-Annexin-V法检测细胞凋亡情况;用试剂盒检测细胞中超氧化物岐化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性以及丙二醛(malondialdehyde,MDA)含量,判断细胞氧化损伤情况。不同浓度Nano-Cd S处理细胞24 h之后,细胞存活率随剂量的增加而下降,浓度为10、20、40和80μg·L~(-1)时,存活率分别为(88.71%±0.80%)、(81.93%±3.06%)、(75.23%±1.13%)和(70.66%±5.63%),且各组间差异均具有统计学意义(P0.05)。以浓度为10和20 mg·L~(-1)的Nano-Cd S染毒24 h后,胞内ROS含量和细胞凋亡率随染毒剂量的增加而增加(P0.05);浓度为10 mg·L~(-1)时,细胞凋亡率为(6.26%±0.44%)。与对照相比,各染毒组SOD和CAT活性和MDA含量升高,20 mg·L~(-1)染毒组SOD和CAT活性和MDA含量高于10 mg·L~(-1)染毒组(P0.05)。研究表明,纳米硫化镉能引起A549细胞的氧化损伤和细胞凋亡,具有明显的细胞毒性。
[Abstract]:The toxicity and oxidative damage effect of nano cadmium sulfide (Nano-Cd S) on lung cancer cell line A549 was studied. A549 cells were cultured and inoculated into 6 orifice plates after subpassages, 2 m L per pore, and infected on the next day. Nano-Cd S, diameter 20~30 nm, and 80~100 nm length were poisoned respectively. After 24 h, the cell survival rate was detected by MTT and the concentration of the survival rate was about 80%. The flow cytometry was used to detect the content of reactive oxygen species (ROS) in A549 cells by fluorescence probe, and the cell apoptosis was detected by PI-Annexin-V method, and the superoxide dismutase (SOD) in the cell was detected by the kit. Superoxide dismutase, SOD) and the activity of catalase (catalase, CAT) and the content of malondialdehyde (malondialdehyde, MDA) were used to determine the oxidative damage of cells. After 24 h of Nano-Cd S treated cells with different concentrations, the survival rate of cells decreased with the increase of dosage, and the survival rate was (88.71% + 0.80%), respectively (81.. 0.80%), respectively, (81.). 93% + 3.06%), (75.23% + 1.13%) and (70.66% + 5.63%), and the differences in each group were statistically significant (P0.05). After Nano-Cd S with 10 and 20 mg. L~ (-1) of 24 h, the intracellular ROS content and cell apoptosis rate increased with the increase of the dose. When the concentration was 10 mg L~ (-1), the cell apoptosis rate was (6.26% + 0.44%). The activity of SOD and CAT and the content of MDA in each group were increased. The activity and MDA content of SOD and CAT in the 20 mg. L~ (-1) group were higher than that of the 10 mg. L~ (-1).

【作者单位】：华北理工大学公共卫生学院河北省煤矿安全与卫生实验室;
【基金】：华北理工大学青年基金项目(Z201425) 大学生创新创业训练计划项目(X2016094)
【分类号】：R114

【相似文献】